DOI: 10.1042/bj3370179. Association of protein-tyrosine phosphatase PTP-BAS with the transcription-factor-inhibitory protein IκBα through interaction between the PDZ1 domain and ankyrin repeats

Association of protein-tyrosine phosphatase PTP-BAS with the transcription-factor-inhibitory protein IκBα through interaction between the PDZ1 domain and ankyrin repeats

10.1042/bj3370179

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

PTP-BAS is a membrane-associated protein tyrosine phosphatase containing a band-4.1 homology region and five PDZ (PSD-95 Dlg ZO-1) [discs-large homology region (‘DHR’)/Gly-Leu-Gly-Phe (‘GLGF’)] domains. The second and fourth PDZ domains were reported to associate with Fas/CD95. By using the first PDZ domain as a bait in yeast two-hybrid screening, we have identified IκBα as a binding protein. IκBα associated with PDZ1 through the stretch of the N-terminal three ankyrin repeats. The association was also confirmed in HeLa cells by co-immunoprecipitation experiments. Inhibition of PTP-BAS by expression of dominant-negative PTP-BAS mutant resulted in tyrosine-phosphorylation of IκBα. Tyrosine-phosphorylation of IκBα is a key event in activation of nuclear factor (NF)-κB during reoxygenation. PTP-BAS may thus play a regulatory role in activation of NF-κB under high oxidative stress.

Bibliography

MAEKAWA, K., IMAGAWA, N., NAITO, A., HARADA, S., YOSHIE, O., & TAKAGI, S. (1999). Association of protein-tyrosine phosphatase PTP-BAS with the transcription-factor-inhibitory protein IÎºBÎ± through interaction between the PDZ1 domain and ankyrin repeats. Biochemical Journal, 337(2), 179â184.

Authors 6

Kazuhiko MAEKAWA (first)
Noriko IMAGAWA (additional)
Akira NAITO (additional)
Shigenori HARADA (additional)
Osamu YOSHIE (additional)
Shin TAKAGI (additional)

References 0 Referenced 49

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 6:04 p.m.)
Deposited	3 years, 9 months ago (Nov. 23, 2021, 7:40 p.m.)
Indexed	2 months, 2 weeks ago (June 21, 2025, 12:48 a.m.)
Issued	26 years, 7 months ago (Jan. 8, 1999)
Published	26 years, 7 months ago (Jan. 8, 1999)
Published Online	26 years, 7 months ago (Jan. 8, 1999)
Published Print	26 years, 7 months ago (Jan. 15, 1999)

Funders 0

None

BibTeX

@article{MAEKAWA_1999, title={Association of protein-tyrosine phosphatase PTP-BAS with the transcription-factor-inhibitory protein IκBα through interaction between the PDZ1 domain and ankyrin repeats}, volume={337}, ISSN={1470-8728}, url={http://dx.doi.org/10.1042/bj3370179}, DOI={10.1042/bj3370179}, number={2}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={MAEKAWA, Kazuhiko and IMAGAWA, Noriko and NAITO, Akira and HARADA, Shigenori and YOSHIE, Osamu and TAKAGI, Shin}, year={1999}, month=jan, pages={179–184} }

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