DOI: 10.1042/bj3280517. Formation of the NO donors glyceryl mononitrate and glyceryl mononitrite from the reaction of peroxynitrite with glycerol

Formation of the NO donors glyceryl mononitrate and glyceryl mononitrite from the reaction of peroxynitrite with glycerol

10.1042/bj3280517

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

Peroxynitrite (ONOO-), formed from the rapid reaction of superoxide (O2-•) with NO, is known to generate stable compounds capable of donating NO on reaction with thiols and molecules containing hydroxy groups. Using glycerol as a model compound for the reactions of ONOO- with biomolecules containing hydroxy groups, we separated the products and identified them by HPLC/MS. It was shown that both glyceryl mononitrate and glyceryl mononitrite were formed and released NO on incubation with copper and L-cysteine. The compounds were stable over a period of 4 h when shielded from light and kept on ice. Slow spontaneous decomposition occurred in the buffer used for the bioassay, but this was not sufficient to explain the vasorelaxing properties of these NO donors. It is concluded that the stable organic nitrate and nitrite have the capacity to be metabolized by vascular tissues, resulting in vasorelaxation.

Bibliography

WHITE, C. R., MOELLERING, D., PATEL, P. R., KIRK, M., BARNES, S., & DARLEY-USMAR, V. M. (1997). Formation of the NO donors glyceryl mononitrate and glyceryl mononitrite from the reaction of peroxynitrite with glycerol. Biochemical Journal, 328(2), 517â524.

Authors 6

C. Roger WHITE (first)
Doug MOELLERING (additional)
P. Rakesh PATEL (additional)
Marion KIRK (additional)
Stephen BARNES (additional)
Victor M. DARLEY-USMAR (additional)

References 0 Referenced 27

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 5:57 p.m.)
Deposited	3 years, 9 months ago (Nov. 23, 2021, 7:38 p.m.)
Indexed	1 year, 10 months ago (Oct. 30, 2023, 10:24 p.m.)
Issued	27 years, 9 months ago (Dec. 1, 1997)
Published	27 years, 9 months ago (Dec. 1, 1997)
Published Print	27 years, 9 months ago (Dec. 1, 1997)

Funders 0

None

BibTeX

@article{WHITE_1997, title={Formation of the NO donors glyceryl mononitrate and glyceryl mononitrite from the reaction of peroxynitrite with glycerol}, volume={328}, ISSN={1470-8728}, url={http://dx.doi.org/10.1042/bj3280517}, DOI={10.1042/bj3280517}, number={2}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={WHITE, C. Roger and MOELLERING, Doug and PATEL, P. Rakesh and KIRK, Marion and BARNES, Stephen and DARLEY-USMAR, Victor M.}, year={1997}, month=dec, pages={517–524} }

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