DOI: 10.1042/bj3250303. Platelet-derived growth factor activation of mitogen-activated protein kinase depends on the sequential activation of phosphatidylcholine-specific phospholipase C, protein kinase C-ζ and Raf-1

Platelet-derived growth factor activation of mitogen-activated protein kinase depends on the sequential activation of phosphatidylcholine-specific phospholipase C, protein kinase C-ζ and Raf-1

10.1042/bj3250303

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

The mechanism of Raf-1 activation by platelet-derived growth factor (PDGF) is poorly defined. We previously reported that, in Rat-1 fibroblasts, PDGF activates a phosphatidylcholine-specific phospholipase C (PC-PLC) and that the product, diacylglycerol, somehow activates protein kinase C-ζ (PKC-ζ). Both PC-PLC and PKC-ζ activities were required for PDGF activation of mitogen-activated protein kinase (MAPK). Now we report that MAPK activation by exogenous PC-PLC depends on Raf-1 activation. PKC-ζ co-immunoprecipitates with, phoshorylates and activates Raf-1, suggesting that in the PDGF- and PC-PLC-activated MAPK pathway, PKC-ζ operates in a signalling complex as a direct activator of Raf-1.

Bibliography

VAN DIJK, M. C. M., HILKMANN, H., & VAN BLITTERSWIJK, W. J. (1997). Platelet-derived growth factor activation of mitogen-activated protein kinase depends on the sequential activation of phosphatidylcholine-specific phospholipase C, protein kinase C-Î¶ and Raf-1. Biochemical Journal, 325(2), 303â307.

Authors 3

Marc C. M. VAN DIJK (first)
Henk HILKMANN (additional)
Wim J. VAN BLITTERSWIJK (additional)

References 0 Referenced 54

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 5:55 p.m.)
Deposited	3 years, 9 months ago (Nov. 23, 2021, 7:16 p.m.)
Indexed	2 months, 3 weeks ago (June 11, 2025, 5:30 a.m.)
Issued	28 years, 1 month ago (July 15, 1997)
Published	28 years, 1 month ago (July 15, 1997)
Published Print	28 years, 1 month ago (July 15, 1997)

Funders 0

None

BibTeX

@article{VAN_DIJK_1997, title={Platelet-derived growth factor activation of mitogen-activated protein kinase depends on the sequential activation of phosphatidylcholine-specific phospholipase C, protein kinase C-ζ and Raf-1}, volume={325}, ISSN={1470-8728}, url={http://dx.doi.org/10.1042/bj3250303}, DOI={10.1042/bj3250303}, number={2}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={VAN DIJK, Marc C. M. and HILKMANN, Henk and VAN BLITTERSWIJK, Wim J.}, year={1997}, month=jul, pages={303–307} }

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