DOI: 10.1042/bj3160073. Biosynthesis of isoprenoids (carotenoids, sterols, prenyl side-chains of chlorophylls and plastoquinone) via a novel pyruvate/glyceraldehyde 3-phosphate non-mevalonate pathway in the green alga <i>Scenedesmus obliquus</i>

Biosynthesis of isoprenoids (carotenoids, sterols, prenyl side-chains of chlorophylls and plastoquinone) via a novel pyruvate/glyceraldehyde 3-phosphate non-mevalonate pathway in the green alga Scenedesmus obliquus

10.1042/bj3160073

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

Isoprenoid biosynthesis was investigated in the green alga Scenedesmus obliquus grown heterotrophically on 13C-labelled glucose and acetate. Several isoprenoid compounds were isolated and investigated by 13C-NMR spectroscopy. According to the 13C-labelling pattern indicated by the 13C-NMR spectra, the biosynthesis of all plastidic isoprenoids investigated (prenyl side-chains of chlorophylls and plastoquinone-9, and the carotenoids β-carotene and lutein), as well as of the non-plastidic cytoplasmic sterols, does not proceed via the classical acetate/mevalonate pathway (which leads from acetyl-CoA via mevalonate to isopentenyl diphosphate), but via the novel glyceraldehyde 3-phosphate/pyruvate route recently detected in eubacteria. Formation of isopentenyl diphosphate involves the condensation of a C2 unit derived from pyruvate decarboxylation with glyceraldehyde 3-phosphate and a transposition yielding the branched C5 skeleton of isoprenic units.

Bibliography

SCHWENDER, J., SEEMANN, M., LICHTENTHALER, H. K., & ROHMER, M. (1996). Biosynthesis of isoprenoids (carotenoids, sterols, prenyl side-chains of chlorophylls and plastoquinone) via a novel pyruvate/glyceraldehyde 3-phosphate non-mevalonate pathway in the green alga Scenedesmus obliquus. Biochemical Journal, 316(1), 73â80.

Authors 4

Jörg SCHWENDER (first)
Myriam SEEMANN (additional)
Hartmut K. LICHTENTHALER (additional)
Michel ROHMER (additional)

References 0 Referenced 304

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 5:49 p.m.)
Deposited	3 years, 9 months ago (Nov. 23, 2021, 7:04 p.m.)
Indexed	1 week, 6 days ago (Aug. 23, 2025, 9:47 p.m.)
Issued	29 years, 3 months ago (May 15, 1996)
Published	29 years, 3 months ago (May 15, 1996)
Published Print	29 years, 3 months ago (May 15, 1996)

Funders 0

None

BibTeX

@article{SCHWENDER_1996, title={Biosynthesis of isoprenoids (carotenoids, sterols, prenyl side-chains of chlorophylls and plastoquinone) via a novel pyruvate/glyceraldehyde 3-phosphate non-mevalonate pathway in the green alga Scenedesmus obliquus}, volume={316}, ISSN={1470-8728}, url={http://dx.doi.org/10.1042/bj3160073}, DOI={10.1042/bj3160073}, number={1}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={SCHWENDER, Jörg and SEEMANN, Myriam and LICHTENTHALER, Hartmut K. and ROHMER, Michel}, year={1996}, month=may, pages={73–80} }

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