DOI: 10.1042/bj3130245. PEST motifs are not required for rapid calpain-mediated proteolysis of c-fos protein

PEST motifs are not required for rapid calpain-mediated proteolysis of c-fos protein

10.1042/bj3130245

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

Cytoplasmic degradation of c-fos protein is extremely rapid. Under certain conditions, it is a multi-step process initiated by calcium-dependent and ATP-independent proteases called calpains. PEST motifs are peptide regions rich in proline, glutamic acid/aspartic acid and serine/threonine residues, commonly assumed to constitute built-in signals for rapid recognition by intracellular proteases and particularly by calpains. Using a cell-free degradation assay and site-directed mutagenesis, we report here that the three PEST motifs of c-fos are not required for rapid cleavage by calpains. Testing the susceptibility of PEST motif-bearing and non-bearing transcription factors including GATA1, GATA3, Myo D, c-erbA, Tal-1 and Sry, demonstrates that PEST sequences are neither necessary nor sufficient for specifying degradation of other proteins by calpains. This conclusion is strengthened by the observation that certain proteins, reportedly known to be cleavable by calpains, are devoid of PEST motifs

Bibliography

CARILLO, S., PARIAT, M., STEFF, A.-M., JARIEL-ENCONTRE, I., POULAT, F., BERTA, P., & PIECHACZYK, M. (1996). PEST motifs are not required for rapid calpain-mediated proteolysis of c-fos protein. Biochemical Journal, 313(1), 245â251.

Authors 7

Serge CARILLO (first)
Magali PARIAT (additional)
Ann-Muriel STEFF (additional)
Isabelle JARIEL-ENCONTRE (additional)
Francis POULAT (additional)
Philippe BERTA (additional)
Marc PIECHACZYK (additional)

References 0 Referenced 42

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 5:47 p.m.)
Deposited	3 years, 9 months ago (Nov. 23, 2021, 8:01 p.m.)
Indexed	3 months, 1 week ago (May 22, 2025, 5:31 a.m.)
Issued	29 years, 7 months ago (Jan. 1, 1996)
Published	29 years, 7 months ago (Jan. 1, 1996)
Published Print	29 years, 7 months ago (Jan. 1, 1996)

Funders 0

None

BibTeX

@article{CARILLO_1996, title={PEST motifs are not required for rapid calpain-mediated proteolysis of c-fos protein}, volume={313}, ISSN={1470-8728}, url={http://dx.doi.org/10.1042/bj3130245}, DOI={10.1042/bj3130245}, number={1}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={CARILLO, Serge and PARIAT, Magali and STEFF, Ann-Muriel and JARIEL-ENCONTRE, Isabelle and POULAT, Francis and BERTA, Philippe and PIECHACZYK, Marc}, year={1996}, month=jan, pages={245–251} }

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