DOI: 10.1042/bj2930275. Purification and characterization of NAD+:ADP-ribosyltransferase (polymerizing) from <i>Dictyostelium discoideum</i>

Purification and characterization of NAD+:ADP-ribosyltransferase (polymerizing) from Dictyostelium discoideum

10.1042/bj2930275

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

A novel affinity-purification scheme based on the tight binding of NAD+:ADP-ribosyltransferase (polymerizing) [pADPRT; poly(ADP-ribose) polymerase; EC 2.4.2.30] to single-strand nicks in DNA, single-stranded patches and DNA ends has been developed to facilitate the purification of this enzyme from the lower eukaryote Dictyostelium discoideum. Two homogeneous forms of the enzyme, with M(r) values of 116,000 and 90,000, were prepared from D. discoideum by using poly(A) hybridized to oligo(dT)-cellulose as affinity material. The Km is 20 microM NAD+ for the 90,000-M(r) protein and 77 microM NAD+ for the 116,000-M(r) protein. The optimum conditions for the enzyme activity in vitro are 6-10 degrees C and pH 8. The time course is linear during the first 10 min of the reaction only. As in enzymes of higher eukaryotes, the activity is dependent on DNA and histone H1 and is inhibited by 3-methoxybenzamide, nicotinamide, theophylline, caffeine and thymidine.

Bibliography

Kofler, B., Wallraff, E., Herzog, H., Schneider, R., Auer, B., & Schweiger, M. (1993). Purification and characterization of NAD+:ADP-ribosyltransferase (polymerizing) from Dictyostelium discoideum. Biochemical Journal, 293(1), 275â281.

Authors 6

B Kofler (first)
E Wallraff (additional)
H Herzog (additional)
R Schneider (additional)
B Auer (additional)
M Schweiger (additional)

References 0 Referenced 19

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 5:33 p.m.)
Deposited	3 years, 9 months ago (Nov. 23, 2021, 5:33 p.m.)
Indexed	1 year, 11 months ago (Sept. 16, 2023, 2:07 p.m.)
Issued	32 years, 1 month ago (July 1, 1993)
Published	32 years, 1 month ago (July 1, 1993)
Published Print	32 years, 1 month ago (July 1, 1993)

Funders 0

None

BibTeX

@article{Kofler_1993, title={Purification and characterization of NAD+:ADP-ribosyltransferase (polymerizing) from Dictyostelium discoideum}, volume={293}, ISSN={1470-8728}, url={http://dx.doi.org/10.1042/bj2930275}, DOI={10.1042/bj2930275}, number={1}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={Kofler, B and Wallraff, E and Herzog, H and Schneider, R and Auer, B and Schweiger, M}, year={1993}, month=jul, pages={275–281} }

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