DOI: 10.1042/bj2920643. Sub-second oscillations of inositol 1,4,5-trisphosphate and inositol 1,3,4,5-tetrakisphosphate during platelet activation by ADP and thrombin: lack of correlation with calcium kinetics

Sub-second oscillations of inositol 1,4,5-trisphosphate and inositol 1,3,4,5-tetrakisphosphate during platelet activation by ADP and thrombin: lack of correlation with calcium kinetics

10.1042/bj2920643

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

The hypothesis that ADP and thrombin liberate Ins(1,4,5)P3 in blood platelets, with kinetics consistent for releasing Ca2+ within 2s, was tested by quenched-flow techniques. Both agonists stimulated transient and equal synthesis of Ins(1,4,5)P3 and Ins(1,3,4,5)P4 near 200 ms and later short-lived peaks, which were not correlated with the slower steady increase in intracellular [Ca2+] between 0.5 to 2 s detected by Indo-1. Shear forces alone caused transient liberation of these inositol phosphates within 0.5 s and up to 4 s, yet failed to increase intracellular [Ca2+].

Bibliography

Raha, S., Jones, G. D., & Gear, A. R. L. (1993). Sub-second oscillations of inositol 1,4,5-trisphosphate and inositol 1,3,4,5-tetrakisphosphate during platelet activation by ADP and thrombin: lack of correlation with calcium kinetics. Biochemical Journal, 292(3), 643â646.

Authors 3

S Raha (first)
G D Jones (additional)
A R L Gear (additional)

References 0 Referenced 30

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 5:32 p.m.)
Deposited	3 years, 9 months ago (Nov. 23, 2021, 5:38 p.m.)
Indexed	3 months, 2 weeks ago (May 20, 2025, 9:32 a.m.)
Issued	32 years, 2 months ago (June 15, 1993)
Published	32 years, 2 months ago (June 15, 1993)
Published Print	32 years, 2 months ago (June 15, 1993)

Funders 0

None

BibTeX

@article{Raha_1993, title={Sub-second oscillations of inositol 1,4,5-trisphosphate and inositol 1,3,4,5-tetrakisphosphate during platelet activation by ADP and thrombin: lack of correlation with calcium kinetics}, volume={292}, ISSN={1470-8728}, url={http://dx.doi.org/10.1042/bj2920643}, DOI={10.1042/bj2920643}, number={3}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={Raha, S and Jones, G D and Gear, A R L}, year={1993}, month=jun, pages={643–646} }

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