DOI: 10.1042/bj2880637. Purification and characterization of phosphatidylinositol 4-phosphate 5-kinases

Purification and characterization of phosphatidylinositol 4-phosphate 5-kinases

10.1042/bj2880637

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

We detail the purification and characterization of three distinct isoforms of PtdIns4P 5-kinase present in bovine brain. One of these, PtdIns4P 5-kinase C, was purified to apparent homogeneity, and SDS/PAGE analysis demonstrated a single polypeptide and molecular mass 53 KDa. These three isoforms were shown to differ in their kinetic properties, and immunological characterization with an antibody raised to PtdIns4P 5-kinase C demonstrated that this isoform was unrelated to the other two. Furthermore, PtdIns4P 5-kinase C was shown to be the bovine brain homologue of the Type II PtdIns4P 5-kinase previously purified from human erythrocytes [Bazenet, Ruano, Brockman & Anderson (1990) J. Biol. Chem. 265, 18012-18022].

Bibliography

Divecha, N., Brooksbank, C. E. L., & Irvine, R. F. (1992). Purification and characterization of phosphatidylinositol 4-phosphate 5-kinases. Biochemical Journal, 288(2), 637â642.

Authors 3

N Divecha (first)
C E L Brooksbank (additional)
R F Irvine (additional)

References 0 Referenced 20

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 5:29 p.m.)
Deposited	3 years, 9 months ago (Nov. 23, 2021, 5:15 p.m.)
Indexed	11 months ago (Oct. 5, 2024, 8:46 p.m.)
Issued	32 years, 9 months ago (Dec. 1, 1992)
Published	32 years, 9 months ago (Dec. 1, 1992)
Published Print	32 years, 9 months ago (Dec. 1, 1992)

Funders 0

None

BibTeX

@article{Divecha_1992, title={Purification and characterization of phosphatidylinositol 4-phosphate 5-kinases}, volume={288}, ISSN={1470-8728}, url={http://dx.doi.org/10.1042/bj2880637}, DOI={10.1042/bj2880637}, number={2}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={Divecha, N and Brooksbank, C E L and Irvine, R F}, year={1992}, month=dec, pages={637–642} }

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