DOI: 10.1042/bj2750435. Inhibition of high-affinity gamma-aminobutyric acid uptake in primary astrocyte cultures by phorbol esters and phospholipase C

Inhibition of high-affinity gamma-aminobutyric acid uptake in primary astrocyte cultures by phorbol esters and phospholipase C

10.1042/bj2750435

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

The effects of phorbol 12-myristate 13-acetate (PMA), a potent activator of protein kinase C (PKC), on high-affinity Na(+)-dependent gamma-aminobutyric acid (GABA) uptake were investigated in primary cultures of neurons and glial cells from rat brain cortex. Incubation of glial cells with PMA led to concentration- and time-dependent decreases in the GABA transport in glial cells. This effect could be completely suppressed by addition of the PKC inhibitor H7. The PMA effects could be mimicked by oleoylacetylglycerol, the diacylglycerol kinase inhibitor R59022 and exogenous phospholipase C. Treatment with PMA did not affect GABA transport in neuronal cells.

Bibliography

Gomeza, J., Casado, M., Gimenez, C., & Aragon, C. (1991). Inhibition of high-affinity gamma-aminobutyric acid uptake in primary astrocyte cultures by phorbol esters and phospholipase C. Biochemical Journal, 275(2), 435â439.

Authors 4

J Gomeza (first)
M Casado (additional)
C Gimenez (additional)
C Aragon (additional)

References 0 Referenced 49

None

Dates

Type	When
Created	10 years ago (Aug. 13, 2015, 5:39 p.m.)
Deposited	3 years, 9 months ago (Nov. 23, 2021, 7:52 p.m.)
Indexed	2 months ago (July 7, 2025, 1:46 a.m.)
Issued	34 years, 4 months ago (April 15, 1991)
Published	34 years, 4 months ago (April 15, 1991)
Published Print	34 years, 4 months ago (April 15, 1991)

Funders 0

None

BibTeX

@article{Gomeza_1991, title={Inhibition of high-affinity gamma-aminobutyric acid uptake in primary astrocyte cultures by phorbol esters and phospholipase C}, volume={275}, ISSN={1470-8728}, url={http://dx.doi.org/10.1042/bj2750435}, DOI={10.1042/bj2750435}, number={2}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={Gomeza, J and Casado, M and Gimenez, C and Aragon, C}, year={1991}, month=apr, pages={435–439} }

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