DOI: 10.1042/bj2680529. A comparative study of the <i>N</i>-linked oligosaccharide structures of human IgG subclass proteins

A comparative study of the N-linked oligosaccharide structures of human IgG subclass proteins

10.1042/bj2680529

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

Quantitative oligosaccharide profiles were determined for each of 18 human IgG paraproteins representing the four subclasses. Each paraprotein exhibits a unique profile that may be substantially different from that observed for polyclonal IgG. The IgG2 and some IgG3 proteins analysed exhibit a predominance of oligosaccharide moieties having galactose on the Man(alpha 1----3) arm rather than the Man(alpha 1----6) arm; it was previously held that galactosylation of the Man(alpha 1----6) arm is preferred, as observed for IgG1, IgG4 and polyclonal IgG. An IgG4 protein is reported that has galactosylated Man(alpha 1----3) and Man(alpha 1----6) arms on both Fc-localized carbohydrate moieties; previous findings suggested that such fully glycosylated structures could not be accommodated within the internal space of the C gamma 2 domains. Unusual monoantennary oligosaccharides present in IgG2 and IgG3 proteins were isolated and their structures determined.

Bibliography

Jefferis, R., Lund, J., Mizutani, H., Nakagawa, H., Kawazoe, Y., Arata, Y., & Takahashi, N. (1990). A comparative study of the N-linked oligosaccharide structures of human IgG subclass proteins. Biochemical Journal, 268(3), 529â537.

Authors 7

R Jefferis (first)
J Lund (additional)
H Mizutani (additional)
H Nakagawa (additional)
Y Kawazoe (additional)
Y Arata (additional)
N Takahashi (additional)

References 0 Referenced 138

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 5:16 p.m.)
Deposited	3 years, 9 months ago (Nov. 25, 2021, 1:37 p.m.)
Indexed	3 weeks, 6 days ago (Aug. 7, 2025, 5:02 a.m.)
Issued	35 years, 2 months ago (June 15, 1990)
Published	35 years, 2 months ago (June 15, 1990)
Published Print	35 years, 2 months ago (June 15, 1990)

Funders 0

None

BibTeX

@article{Jefferis_1990, title={A comparative study of the N-linked oligosaccharide structures of human IgG subclass proteins}, volume={268}, ISSN={1470-8728}, url={http://dx.doi.org/10.1042/bj2680529}, DOI={10.1042/bj2680529}, number={3}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={Jefferis, R and Lund, J and Mizutani, H and Nakagawa, H and Kawazoe, Y and Arata, Y and Takahashi, N}, year={1990}, month=jun, pages={529–537} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        7
      ]
    ],
    "date-time": "2025-08-07T09:02:50Z",
    "timestamp": 1754557370470
  },
  "reference-count": 0,
  "publisher": "Portland Press Ltd.",
  "issue": "3",
  "content-domain": {
    "domain": [
      "portlandpress.com"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1990,
        6,
        15
      ]
    ]
  },
  "abstract": "<jats:p>Quantitative oligosaccharide profiles were determined for each of 18 human IgG paraproteins representing the four subclasses. Each paraprotein exhibits a unique profile that may be substantially different from that observed for polyclonal IgG. The IgG2 and some IgG3 proteins analysed exhibit a predominance of oligosaccharide moieties having galactose on the Man(alpha 1----3) arm rather than the Man(alpha 1----6) arm; it was previously held that galactosylation of the Man(alpha 1----6) arm is preferred, as observed for IgG1, IgG4 and polyclonal IgG. An IgG4 protein is reported that has galactosylated Man(alpha 1----3) and Man(alpha 1----6) arms on both Fc-localized carbohydrate moieties; previous findings suggested that such fully glycosylated structures could not be accommodated within the internal space of the C gamma 2 domains. Unusual monoantennary oligosaccharides present in IgG2 and IgG3 proteins were isolated and their structures determined.</jats:p>",
  "DOI": "10.1042/bj2680529",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2015,
        8,
        10
      ]
    ],
    "date-time": "2015-08-10T21:16:34Z",
    "timestamp": 1439241394000
  },
  "page": "529-537",
  "update-policy": "http://dx.doi.org/10.1042/crossmark_policy",
  "source": "Crossref",
  "is-referenced-by-count": 138,
  "title": "A comparative study of the <i>N</i>-linked oligosaccharide structures of human IgG subclass proteins",
  "prefix": "10.1042",
  "volume": "268",
  "author": [
    {
      "given": "R",
      "family": "Jefferis",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Immunology, University of Birmingham Medical School, Vincent Drive, Edgbaston, Birmingham B15 2TJ, U.K."
        }
      ]
    },
    {
      "given": "J",
      "family": "Lund",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Immunology, University of Birmingham Medical School, Vincent Drive, Edgbaston, Birmingham B15 2TJ, U.K."
        }
      ]
    },
    {
      "given": "H",
      "family": "Mizutani",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Faculty of Pharmaceutical Sciences, Nagoya City University, Mizuho-ku, Nagoya 467, Japan"
        }
      ]
    },
    {
      "given": "H",
      "family": "Nakagawa",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Faculty of Pharmaceutical Sciences, Nagoya City University, Mizuho-ku, Nagoya 467, Japan"
        }
      ]
    },
    {
      "given": "Y",
      "family": "Kawazoe",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Faculty of Pharmaceutical Sciences, Nagoya City University, Mizuho-ku, Nagoya 467, Japan"
        }
      ]
    },
    {
      "given": "Y",
      "family": "Arata",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "University of Tokyo, Hongo, Tokyo 113, Japan"
        }
      ]
    },
    {
      "given": "N",
      "family": "Takahashi",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Nagoya City University College of Nursing, Mizuho-ku, Nagoya 467, Japan"
        }
      ]
    }
  ],
  "member": "288",
  "container-title": "Biochemical Journal",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://portlandpress.com/biochemj/article-pdf/268/3/529/599348/bj2680529.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://portlandpress.com/biochemj/article-pdf/268/3/529/599348/bj2680529.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        11,
        25
      ]
    ],
    "date-time": "2021-11-25T18:37:32Z",
    "timestamp": 1637865452000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://portlandpress.com/biochemj/article/268/3/529/26297/A-comparative-study-of-the-N-linked"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1990,
        6,
        15
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          1990,
          6,
          15
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1042/bj2680529",
  "relation": {},
  "ISSN": [
    "0264-6021",
    "1470-8728"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1990,
        6,
        15
      ]
    ]
  }
}