DOI: 10.1042/bj2670413. Effect of gangliosides on membrane permeability studied by enzymic and fluorescence-spectroscopy techniques

Effect of gangliosides on membrane permeability studied by enzymic and fluorescence-spectroscopy techniques

10.1042/bj2670413

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

The effect of gangliosides on membrane permeability was investigated by studying the kinetic properties of cytochrome c oxidase, the activity of which, when the enzyme is reconstituted in phospholipid vesicles, is dependent on membrane permeability to H+ and K+. The experiments indicate that three different gangliosides (GM1, DD1a, GT1b) incorporated into cytochrome c oxidase-containing phospholipid vesicles stimulate enzymic activity, in the absence of ionophores, most probably by disorganizing the bilayer lipid assembly and increasing its permeability to ions. This interpretation was confirmed by fluorescence-spectroscopy experiments in which the rate of passive leakage of carboxyfluorescein entrapped in the vesicles was measured. Cholera toxin, or its isolated B-subunit, added to GM1-containing proteoliposomes inhibited cytochrome c oxidase activity, indicating the lack of formation, under these experimental conditions, of channels freely permeable to H+ or K+.

Bibliography

Sarti, P., Antonini, G., Malatesta, F., Vallone, B., Brunori, M., Masserini, M., Palestini, P., & Tettamanti, G. (1990). Effect of gangliosides on membrane permeability studied by enzymic and fluorescence-spectroscopy techniques. Biochemical Journal, 267(2), 413â416.

Authors 8

P Sarti (first)
G Antonini (additional)
F Malatesta (additional)
B Vallone (additional)
M Brunori (additional)
M Masserini (additional)
P Palestini (additional)
G Tettamanti (additional)

References 0 Referenced 20

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 25, 2021, 1:21 p.m.)
Indexed	1 year, 6 months ago (Feb. 7, 2024, 2:32 a.m.)
Issued	35 years, 4 months ago (April 15, 1990)
Published	35 years, 4 months ago (April 15, 1990)
Published Print	35 years, 4 months ago (April 15, 1990)

Funders 0

None

BibTeX

@article{Sarti_1990, title={Effect of gangliosides on membrane permeability studied by enzymic and fluorescence-spectroscopy techniques}, volume={267}, ISSN={1470-8728}, url={http://dx.doi.org/10.1042/bj2670413}, DOI={10.1042/bj2670413}, number={2}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={Sarti, P and Antonini, G and Malatesta, F and Vallone, B and Brunori, M and Masserini, M and Palestini, P and Tettamanti, G}, year={1990}, month=apr, pages={413–416} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        2,
        7
      ]
    ],
    "date-time": "2024-02-07T07:32:58Z",
    "timestamp": 1707291178700
  },
  "reference-count": 0,
  "publisher": "Portland Press Ltd.",
  "issue": "2",
  "content-domain": {
    "domain": [
      "portlandpress.com"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1990,
        4,
        15
      ]
    ]
  },
  "abstract": "<jats:p>The effect of gangliosides on membrane permeability was investigated by studying the kinetic properties of cytochrome c oxidase, the activity of which, when the enzyme is reconstituted in phospholipid vesicles, is dependent on membrane permeability to H+ and K+. The experiments indicate that three different gangliosides (GM1, DD1a, GT1b) incorporated into cytochrome c oxidase-containing phospholipid vesicles stimulate enzymic activity, in the absence of ionophores, most probably by disorganizing the bilayer lipid assembly and increasing its permeability to ions. This interpretation was confirmed by fluorescence-spectroscopy experiments in which the rate of passive leakage of carboxyfluorescein entrapped in the vesicles was measured. Cholera toxin, or its isolated B-subunit, added to GM1-containing proteoliposomes inhibited cytochrome c oxidase activity, indicating the lack of formation, under these experimental conditions, of channels freely permeable to H+ or K+.</jats:p>",
  "DOI": "10.1042/bj2670413",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2015,
        8,
        10
      ]
    ],
    "date-time": "2015-08-10T21:15:42Z",
    "timestamp": 1439241342000
  },
  "page": "413-416",
  "update-policy": "http://dx.doi.org/10.1042/crossmark_policy",
  "source": "Crossref",
  "is-referenced-by-count": 20,
  "title": "Effect of gangliosides on membrane permeability studied by enzymic and fluorescence-spectroscopy techniques",
  "prefix": "10.1042",
  "volume": "267",
  "author": [
    {
      "given": "P",
      "family": "Sarti",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Center of Molecular Biology (CNR), University of Rome \u2018Tor Vergata\u2019, Rome, Italy."
        },
        {
          "name": "Department of Biomedical Sciences, Technology and Biometry, University of L'Aquila, L'Aquila, Italy."
        }
      ]
    },
    {
      "given": "G",
      "family": "Antonini",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Experimental Medicine and Biochemical Sciences, University of Rome \u2018Tor Vergata\u2019, Rome, Italy."
        }
      ]
    },
    {
      "given": "F",
      "family": "Malatesta",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Experimental Medicine and Biochemical Sciences, University of Rome \u2018Tor Vergata\u2019, Rome, Italy."
        }
      ]
    },
    {
      "given": "B",
      "family": "Vallone",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Biochemical Sciences, University of Rome \u2018La Sapienza\u2019, Rome, Italy."
        }
      ]
    },
    {
      "given": "M",
      "family": "Brunori",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Biochemical Sciences, University of Rome \u2018La Sapienza\u2019, Rome, Italy."
        }
      ]
    },
    {
      "given": "M",
      "family": "Masserini",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Biochemistry, Medical School, University of Milano, Milano, Italy."
        }
      ]
    },
    {
      "given": "P",
      "family": "Palestini",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Biochemistry, Medical School, University of Milano, Milano, Italy."
        }
      ]
    },
    {
      "given": "G",
      "family": "Tettamanti",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Biochemistry, Medical School, University of Milano, Milano, Italy."
        }
      ]
    }
  ],
  "member": "288",
  "container-title": "Biochemical Journal",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://portlandpress.com/biochemj/article-pdf/267/2/413/599017/bj2670413.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://portlandpress.com/biochemj/article-pdf/267/2/413/599017/bj2670413.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        11,
        25
      ]
    ],
    "date-time": "2021-11-25T18:21:33Z",
    "timestamp": 1637864493000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://portlandpress.com/biochemj/article/267/2/413/25536/Effect-of-gangliosides-on-membrane-permeability"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1990,
        4,
        15
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "2",
    "published-print": {
      "date-parts": [
        [
          1990,
          4,
          15
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1042/bj2670413",
  "relation": {},
  "ISSN": [
    "0264-6021",
    "1470-8728"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1990,
        4,
        15
      ]
    ]
  }
}