DOI: 10.1042/bj2650617. Mass measurement of inositol 1,4,5-trisphosphate and <i>sn</i>-1,2-diacylglycerol in bombesin-stimulated Swiss 3T3 mouse fibroblasts

Mass measurement of inositol 1,4,5-trisphosphate and sn-1,2-diacylglycerol in bombesin-stimulated Swiss 3T3 mouse fibroblasts

10.1042/bj2650617

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

Two specific and selective assays were used to measure changes in the mass of Ins(1,4,5)P3 and sn-1,2-diacylglycerol in bombesin-stimulated Swiss 3T3 cells. The results demonstrate that the increase in Ins(1,4,5)P3 was extremely rapid, but transient, returning to basal levels by 30 s. In contrast, the increase in sn-1,2-diacylglycerol was biphasic: the first phase mirrored the transient Ins(1,4,5)P3 response, whereas the second phase was sustained and occurred in the absence of elevated Ins(1,4,5)P3. The possible source of the second phase of diacylglycerol is discussed.

Bibliography

Cook, S. J., Palmer, S., Plevin, R., & Wakelam, M. J. O. (1990). Mass measurement of inositol 1,4,5-trisphosphate and sn-1,2-diacylglycerol in bombesin-stimulated Swiss 3T3 mouse fibroblasts. Biochemical Journal, 265(2), 617â620.

Authors 4

S J Cook (first)
S Palmer (additional)
R Plevin (additional)
M J O Wakelam (additional)

References 0 Referenced 45

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 5:14 p.m.)
Deposited	3 years, 9 months ago (Nov. 25, 2021, 1:17 p.m.)
Indexed	1 year, 6 months ago (Feb. 20, 2024, 1:02 p.m.)
Issued	35 years, 7 months ago (Jan. 15, 1990)
Published	35 years, 7 months ago (Jan. 15, 1990)
Published Print	35 years, 7 months ago (Jan. 15, 1990)

Funders 0

None

BibTeX

@article{Cook_1990, title={Mass measurement of inositol 1,4,5-trisphosphate and sn-1,2-diacylglycerol in bombesin-stimulated Swiss 3T3 mouse fibroblasts}, volume={265}, ISSN={1470-8728}, url={http://dx.doi.org/10.1042/bj2650617}, DOI={10.1042/bj2650617}, number={2}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={Cook, S J and Palmer, S and Plevin, R and Wakelam, M J O}, year={1990}, month=jan, pages={617–620} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        2,
        20
      ]
    ],
    "date-time": "2024-02-20T18:02:59Z",
    "timestamp": 1708452179615
  },
  "reference-count": 0,
  "publisher": "Portland Press Ltd.",
  "issue": "2",
  "content-domain": {
    "domain": [
      "portlandpress.com"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1990,
        1,
        15
      ]
    ]
  },
  "abstract": "<jats:p>Two specific and selective assays were used to measure changes in the mass of Ins(1,4,5)P3 and sn-1,2-diacylglycerol in bombesin-stimulated Swiss 3T3 cells. The results demonstrate that the increase in Ins(1,4,5)P3 was extremely rapid, but transient, returning to basal levels by 30 s. In contrast, the increase in sn-1,2-diacylglycerol was biphasic: the first phase mirrored the transient Ins(1,4,5)P3 response, whereas the second phase was sustained and occurred in the absence of elevated Ins(1,4,5)P3. The possible source of the second phase of diacylglycerol is discussed.</jats:p>",
  "DOI": "10.1042/bj2650617",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2015,
        8,
        10
      ]
    ],
    "date-time": "2015-08-10T21:14:47Z",
    "timestamp": 1439241287000
  },
  "page": "617-620",
  "update-policy": "http://dx.doi.org/10.1042/crossmark_policy",
  "source": "Crossref",
  "is-referenced-by-count": 45,
  "title": "Mass measurement of inositol 1,4,5-trisphosphate and <i>sn</i>-1,2-diacylglycerol in bombesin-stimulated Swiss 3T3 mouse fibroblasts",
  "prefix": "10.1042",
  "volume": "265",
  "author": [
    {
      "given": "S J",
      "family": "Cook",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Molecular Pharmacology Group, Department of Biochemistry, University of Glasgow, Glasgow G12 8QQ, Scotland, U.K."
        }
      ]
    },
    {
      "given": "S",
      "family": "Palmer",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Molecular Pharmacology Group, Department of Biochemistry, University of Glasgow, Glasgow G12 8QQ, Scotland, U.K."
        }
      ]
    },
    {
      "given": "R",
      "family": "Plevin",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Molecular Pharmacology Group, Department of Biochemistry, University of Glasgow, Glasgow G12 8QQ, Scotland, U.K."
        }
      ]
    },
    {
      "given": "M J O",
      "family": "Wakelam",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Molecular Pharmacology Group, Department of Biochemistry, University of Glasgow, Glasgow G12 8QQ, Scotland, U.K."
        }
      ]
    }
  ],
  "member": "288",
  "container-title": "Biochemical Journal",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://portlandpress.com/biochemj/article-pdf/265/2/617/598740/bj2650617.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://portlandpress.com/biochemj/article-pdf/265/2/617/598740/bj2650617.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        11,
        25
      ]
    ],
    "date-time": "2021-11-25T18:17:58Z",
    "timestamp": 1637864278000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://portlandpress.com/biochemj/article/265/2/617/25230/Mass-measurement-of-inositol-1-4-5-trisphosphate"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1990,
        1,
        15
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "2",
    "published-print": {
      "date-parts": [
        [
          1990,
          1,
          15
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1042/bj2650617",
  "relation": {},
  "ISSN": [
    "0264-6021",
    "1470-8728"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1990,
        1,
        15
      ]
    ]
  }
}