DOI: 10.1042/bj2600395. Evidence for intracellular sodium pumps in permeabilized <i>Xenopus laevis</i> oocytes

Evidence for intracellular sodium pumps in permeabilized Xenopus laevis oocytes

10.1042/bj2600395

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

Ouabain binding was studied in Xenopus laevis oocytes permeabilized by detergents. The behaviour of markers showed that 10 microM-digitonin selectively disrupts the plasma membrane. In the presence of ATP, oocytes permeabilized at 10 microM-digitonin bound no more ouabain molecules than were required to abolish active 86Rb+ uptake in the intact cells. However, the ouabain binding capacity increased approx. 2-fold when inner membranes were disrupted by SDS or excess digitonin, as judged from the accompanying release of the lysosomal marker beta-hexosaminidase. The results suggest that oocytes have a large internal pool of functional sodium pumps.

Bibliography

Schmalzing, G., KrÃ¶ner, S., & Passow, H. (1989). Evidence for intracellular sodium pumps in permeabilized Xenopus laevis oocytes. Biochemical Journal, 260(2), 395â399.

Authors 3

G Schmalzing (first)
S Kröner (additional)
H Passow (additional)

References 0 Referenced 15

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 5:13 p.m.)
Deposited	3 years, 9 months ago (Nov. 25, 2021, 1:13 p.m.)
Indexed	1 year, 2 months ago (June 17, 2024, 4:39 p.m.)
Issued	36 years, 2 months ago (June 1, 1989)
Published	36 years, 2 months ago (June 1, 1989)
Published Print	36 years, 2 months ago (June 1, 1989)

Funders 0

None

BibTeX

@article{Schmalzing_1989, title={Evidence for intracellular sodium pumps in permeabilized Xenopus laevis oocytes}, volume={260}, ISSN={1470-8728}, url={http://dx.doi.org/10.1042/bj2600395}, DOI={10.1042/bj2600395}, number={2}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={Schmalzing, G and Kröner, S and Passow, H}, year={1989}, month=jun, pages={395–399} }

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