DOI: 10.1042/bj2561029. Conversion of a phosphoseryl/threonyl phosphatase into a phosphotyrosyl phosphatase

Conversion of a phosphoseryl/threonyl phosphatase into a phosphotyrosyl phosphatase

10.1042/bj2561029

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

By use of the autophosphorylated epidermal-growth-factor receptor and the synthetic peptide RRLIE-DAEY(P)AARG, representing an autophosphorylation site of the transforming protein of Rous-sarcoma virus, it is demonstrated that the phosphotyrosyl phosphatase activity of the polycation-stimulated phosphatases is substantially increased by an enzyme-directed effect of ATP or PPi. Concomitant with this increase in phosphotyrosyl phosphatase activity, the phosphorylase phosphatase activity is decreased, thus dramatically changing the substrate specificity of these enzymes. The dephosphorylation of four different phosphotyrosyl sites of the epidermal-growth-factor receptor is neither consecutive nor at random, but a preferred dephosphorylation of the P1 site over the P3 greater than P2 greater than P4 sites is observed. This phosphatase activity represents a substantial fraction of the total phosphotyrosyl phosphatase activity in the post-mitochondrial supernatant of Xenopus laevis oocytes.

Bibliography

Goris, J., Pallen, C. J., Parker, P. J., Hermann, J., Waterfield, M. D., & Merlevede, W. (1988). Conversion of a phosphoseryl/threonyl phosphatase into a phosphotyrosyl phosphatase. Biochemical Journal, 256(3), 1029â1034.

Authors 6

J Goris (first)
C J Pallen (additional)
P J Parker (additional)
J Hermann (additional)
M D Waterfield (additional)
W Merlevede (additional)

References 0 Referenced 44

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 5:12 p.m.)
Deposited	3 years, 9 months ago (Nov. 25, 2021, 1:19 p.m.)
Indexed	1 year, 2 months ago (June 17, 2024, 4:40 p.m.)
Issued	36 years, 8 months ago (Dec. 15, 1988)
Published	36 years, 8 months ago (Dec. 15, 1988)
Published Print	36 years, 8 months ago (Dec. 15, 1988)

Funders 0

None

BibTeX

@article{Goris_1988, title={Conversion of a phosphoseryl/threonyl phosphatase into a phosphotyrosyl phosphatase}, volume={256}, ISSN={1470-8728}, url={http://dx.doi.org/10.1042/bj2561029}, DOI={10.1042/bj2561029}, number={3}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={Goris, J and Pallen, C J and Parker, P J and Hermann, J and Waterfield, M D and Merlevede, W}, year={1988}, month=dec, pages={1029–1034} }

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