DOI: 10.1042/bj2530153. Evidence for filaggrin as a component of the cell envelope of the newborn rat

Evidence for filaggrin as a component of the cell envelope of the newborn rat

10.1042/bj2530153

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

A substrate of transglutaminase, specific to the epidermis, was identified, by fluorescent and radioactive labelling with the lysine analogues dansylcadaverine and [14C]putrescine respectively, in newborn-rat epidermal homogenates and whole-skin organ cultures. The labelled analogues were preferentially incorporated into the stratum-corneum protein filaggrin in a Ca2+-dependent manner in both ‘in vitro’ systems. When filaggrin was labelled in vivo with [3H]histidine and then incubated with rat epidermal preparations, the label was rendered SDS/thiol-insoluble. Incorporation of [3H]filaggrin into the insoluble envelope fraction was Ca2+-dependent and inhibited by EDTA and exogenous amines. Antisera to newborn-rat filaggrin cross-reacted with purified newborn-rat cell envelopes, and this reaction was blocked by adsorbing the antiserum with purified filaggrin. Quantification of the ‘envelope-bound’ filaggrin showed it to be a significant component, accounting for approx. 10% of the cell-envelope protein.

Bibliography

Richards, S., Scott, I. R., Harding, C. R., Liddell, J. E., Powell, G. M., & Curtis, C. G. (1988). Evidence for filaggrin as a component of the cell envelope of the newborn rat. Biochemical Journal, 253(1), 153â160.

Authors 6

S Richards (first)
I R Scott (additional)
C R Harding (additional)
J E Liddell (additional)
G M Powell (additional)
C G Curtis (additional)

References 0 Referenced 48

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 5:11 p.m.)
Deposited	3 years, 9 months ago (Nov. 25, 2021, 1:01 p.m.)
Indexed	1 year, 7 months ago (Jan. 16, 2024, 1:22 p.m.)
Issued	37 years, 1 month ago (July 1, 1988)
Published	37 years, 1 month ago (July 1, 1988)
Published Print	37 years, 1 month ago (July 1, 1988)

Funders 0

None

BibTeX

@article{Richards_1988, title={Evidence for filaggrin as a component of the cell envelope of the newborn rat}, volume={253}, ISSN={1470-8728}, url={http://dx.doi.org/10.1042/bj2530153}, DOI={10.1042/bj2530153}, number={1}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={Richards, S and Scott, I R and Harding, C R and Liddell, J E and Powell, G M and Curtis, C G}, year={1988}, month=jul, pages={153–160} }

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