DOI: 10.1042/bj2480489. Turkey erythrocytes possess a membrane-associated inositol 1,4,5-trisphosphate 3-kinase that is activated by Ca2+ in the presence of calmodulin

Turkey erythrocytes possess a membrane-associated inositol 1,4,5-trisphosphate 3-kinase that is activated by Ca2+ in the presence of calmodulin

10.1042/bj2480489

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

Turkey erythrocytes contain soluble and particulate kinase activities which catalyse the ATP-dependent phosphorylation of inositol 1,4,5-trisphosphate [Ins(1,4,5)P3]. The particle-bound activity accounts for approximately one-quarter of the total cellular Ins(1,4,5)P3 kinase, when assayed at a [Ca2+] of 10 nM. The particle-bound Ins(1,4,5)P3 kinase is not washed from the membrane by 0.6 M-KCl, yet may be solubilized by a variety of detergents. This suggests that it is an intrinsic membrane protein. The product of the membrane-bound Ins(1,4,5)P3 kinase is inositol 1,3,4,5-tetrakisphosphate [Ins(1,3,4,5)P4], identifying the enzyme as an Ins(1,4,5)P3 3-kinase. In the presence of calmodulin, the membrane-associated Ins(1,4,5)P3 3-kinase is activated as [Ca2+] is increased over the range 0.2-1.0 microM. Under these conditions, the rates of dephosphorylation of Ins(1,3,4,5)P4 and Ins(1,4,5)P3 by phosphatases in the membrane fraction are unchanged.

Bibliography

Morris, A. J., Downes, C. P., Harden, T. K., & Michell, R. H. (1987). Turkey erythrocytes possess a membrane-associated inositol 1,4,5-trisphosphate 3-kinase that is activated by Ca2+ in the presence of calmodulin. Biochemical Journal, 248(2), 489â493.

Authors 4

A J Morris (first)
C P Downes (additional)
T K Harden (additional)
R H Michell (additional)

References 0 Referenced 39

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 5:09 p.m.)
Deposited	3 years, 9 months ago (Nov. 25, 2021, 12:57 p.m.)
Indexed	1 year, 7 months ago (Jan. 12, 2024, 5:39 a.m.)
Issued	37 years, 9 months ago (Dec. 1, 1987)
Published	37 years, 9 months ago (Dec. 1, 1987)
Published Print	37 years, 9 months ago (Dec. 1, 1987)

Funders 0

None

BibTeX

@article{Morris_1987, title={Turkey erythrocytes possess a membrane-associated inositol 1,4,5-trisphosphate 3-kinase that is activated by Ca2+ in the presence of calmodulin}, volume={248}, ISSN={1470-8728}, url={http://dx.doi.org/10.1042/bj2480489}, DOI={10.1042/bj2480489}, number={2}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={Morris, A J and Downes, C P and Harden, T K and Michell, R H}, year={1987}, month=dec, pages={489–493} }

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