DOI: 10.1042/bj2460619. Phorbol-ester-induced alterations of free calcium ion transients in single rat hepatocytes

Phorbol-ester-induced alterations of free calcium ion transients in single rat hepatocytes

10.1042/bj2460619

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

The effect of the phorbol esters phorbol 12-myristate 13-acetate (TPA) and phorbol 12,13-dibutyrate (PDB) on changes in free Ca2+ concentration ([Ca2+]i) in single rat hepatocytes, microinjected with the photoprotein aequorin, were investigated. [Arg8]vasopressin and phenylephrine induced a series of repetitive [Ca2+]i transients. Phorbol esters inhibited the alpha 1-adrenoceptor-induced response; sub-nanomolar concentrations decreased the transient frequency, and higher concentrations abolished the transients. The inhibitory effect of PDB was readily reversible. Phorbol esters were less effective in decreasing the frequency of [Arg8]-vasopressin-induced transients, and the inhibition could be overcome by high [Arg8]vasopressin concentrations.

Bibliography

Woods, N. M., Cuthbertson, K. S. R., & Cobbold, P. H. (1987). Phorbol-ester-induced alterations of free calcium ion transients in single rat hepatocytes. Biochemical Journal, 246(3), 619â623.

Authors 3

N M Woods (first)
K S R Cuthbertson (additional)
P H Cobbold (additional)

References 0 Referenced 76

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 5:01 p.m.)
Deposited	3 years, 9 months ago (Nov. 25, 2021, 1:22 p.m.)
Indexed	1 year, 2 months ago (June 17, 2024, 4:30 p.m.)
Issued	37 years, 11 months ago (Sept. 15, 1987)
Published	37 years, 11 months ago (Sept. 15, 1987)
Published Print	37 years, 11 months ago (Sept. 15, 1987)

Funders 0

None

BibTeX

@article{Woods_1987, title={Phorbol-ester-induced alterations of free calcium ion transients in single rat hepatocytes}, volume={246}, ISSN={1470-8728}, url={http://dx.doi.org/10.1042/bj2460619}, DOI={10.1042/bj2460619}, number={3}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={Woods, N M and Cuthbertson, K S R and Cobbold, P H}, year={1987}, month=sep, pages={619–623} }

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