DOI: 10.1042/bj2430661. Dihydrolipoamide dehydrogenase from <i>Trypanosoma brucei</i>. Characterization and cellular location

Dihydrolipoamide dehydrogenase from Trypanosoma brucei. Characterization and cellular location

10.1042/bj2430661

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

Dihydrolipoamide dehydrogenase has been discovered in the bloodstream form of the eukaryotic African parasite, Trypanosoma brucei. The enzyme catalysed the stoichiometric oxidation of dihydrolipoamide by NAD+ and exhibited a hyperbolic dependence of catalytic activity on the concentrations of both dihydrolipoamide and NAD+. Chemical modification with the tervalent arsenical reagent p-aminophenyldichloroarsine indicates the involvement in catalysis of a reversibly reducible disulphide bond. Plasma-membrane sheets were purified from T. brucei, and it was shown that virtually all the dihydrolipoamide dehydrogenase remained closely associated with this membrane preparation. T. brucei apparently lacks the 2-oxoacid dehydrogenase multienzyme complexes of which dihydrolipoamide dehydrogenase is usually an integral component. In the context of this absence, the possible function of trypanosomal dihydrolipoamide dehydrogenase is discussed, with particular reference to its cellular location in the plasma membrane.

Bibliography

Danson, M. J., Conroy, K., McQuattie, A., & Stevenson, K. J. (1987). Dihydrolipoamide dehydrogenase from Trypanosoma brucei. Characterization and cellular location. Biochemical Journal, 243(3), 661â665.

Authors 4

M J Danson (first)
K Conroy (additional)
A McQuattie (additional)
K J Stevenson (additional)

References 0 Referenced 60

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 4:58 p.m.)
Deposited	3 years, 8 months ago (Nov. 25, 2021, 12:48 p.m.)
Indexed	2 weeks, 5 days ago (Aug. 5, 2025, 8:59 a.m.)
Issued	38 years, 3 months ago (May 1, 1987)
Published	38 years, 3 months ago (May 1, 1987)
Published Print	38 years, 3 months ago (May 1, 1987)

Funders 0

None

BibTeX

@article{Danson_1987, title={Dihydrolipoamide dehydrogenase from Trypanosoma brucei. Characterization and cellular location}, volume={243}, ISSN={1470-8728}, url={http://dx.doi.org/10.1042/bj2430661}, DOI={10.1042/bj2430661}, number={3}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={Danson, M J and Conroy, K and McQuattie, A and Stevenson, K J}, year={1987}, month=may, pages={661–665} }

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