DOI: 10.1042/bj2400913. Comparison of human stromelysin and collagenase by cloning and sequence analysis

Comparison of human stromelysin and collagenase by cloning and sequence analysis

10.1042/bj2400913

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

A comparison of the cDNA-derived amino acid sequences of human stromelysin and collagenase with the N-terminal sequences of purified enzymes reveals that these metalloproteinases are highly conserved and that they are secreted as proenzymes. A putative zinc-binding site was identified by its homology with the zinc-chelating sequence of thermolysin. These sequences permitted the identification of: transin, a protein induced in rat fibroblasts either exposed to growth factors or transformed by oncogenic viruses, as the rat homologue of stromelysin, and XHF1, a protein induced in human fibroblasts after treatment with tumourigenic agents, as collagenase.

Bibliography

Whitham, S. E., Murphy, G., Angel, P., Rahmsdorf, H. J., Smith, B. J., Lyons, A., Harris, T. J. R., Reynolds, J. J., Herrlich, P., & Docherty, A. J. P. (1986). Comparison of human stromelysin and collagenase by cloning and sequence analysis. Biochemical Journal, 240(3), 913â916.

Authors 10

S E Whitham (first)
G Murphy (additional)
P Angel (additional)
H J Rahmsdorf (additional)
B J Smith (additional)
A Lyons (additional)
T J R Harris (additional)
J J Reynolds (additional)
P Herrlich (additional)
A J P Docherty (additional)

References 0 Referenced 313

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 4:57 p.m.)
Deposited	3 years, 9 months ago (Nov. 25, 2021, 11:53 a.m.)
Indexed	1 month, 4 weeks ago (July 2, 2025, 2:47 p.m.)
Issued	38 years, 8 months ago (Dec. 15, 1986)
Published	38 years, 8 months ago (Dec. 15, 1986)
Published Print	38 years, 8 months ago (Dec. 15, 1986)

Funders 0

None

BibTeX

@article{Whitham_1986, title={Comparison of human stromelysin and collagenase by cloning and sequence analysis}, volume={240}, ISSN={1470-8728}, url={http://dx.doi.org/10.1042/bj2400913}, DOI={10.1042/bj2400913}, number={3}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={Whitham, S E and Murphy, G and Angel, P and Rahmsdorf, H J and Smith, B J and Lyons, A and Harris, T J R and Reynolds, J J and Herrlich, P and Docherty, A J P}, year={1986}, month=dec, pages={913–916} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        2
      ]
    ],
    "date-time": "2025-07-02T18:47:32Z",
    "timestamp": 1751482052702
  },
  "reference-count": 0,
  "publisher": "Portland Press Ltd.",
  "issue": "3",
  "content-domain": {
    "domain": [
      "portlandpress.com"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1986,
        12,
        15
      ]
    ]
  },
  "abstract": "<jats:p>A comparison of the cDNA-derived amino acid sequences of human stromelysin and collagenase with the N-terminal sequences of purified enzymes reveals that these metalloproteinases are highly conserved and that they are secreted as proenzymes. A putative zinc-binding site was identified by its homology with the zinc-chelating sequence of thermolysin. These sequences permitted the identification of: transin, a protein induced in rat fibroblasts either exposed to growth factors or transformed by oncogenic viruses, as the rat homologue of stromelysin, and XHF1, a protein induced in human fibroblasts after treatment with tumourigenic agents, as collagenase.</jats:p>",
  "DOI": "10.1042/bj2400913",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2015,
        8,
        10
      ]
    ],
    "date-time": "2015-08-10T20:57:04Z",
    "timestamp": 1439240224000
  },
  "page": "913-916",
  "update-policy": "http://dx.doi.org/10.1042/crossmark_policy",
  "source": "Crossref",
  "is-referenced-by-count": 313,
  "title": "Comparison of human stromelysin and collagenase by cloning and sequence analysis",
  "prefix": "10.1042",
  "volume": "240",
  "author": [
    {
      "given": "S E",
      "family": "Whitham",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "G",
      "family": "Murphy",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "P",
      "family": "Angel",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "H J",
      "family": "Rahmsdorf",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "B J",
      "family": "Smith",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "A",
      "family": "Lyons",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "T J R",
      "family": "Harris",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "J J",
      "family": "Reynolds",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "P",
      "family": "Herrlich",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "A J P",
      "family": "Docherty",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "288",
  "container-title": "Biochemical Journal",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://portlandpress.com/biochemj/article-pdf/240/3/913/588955/bj2400913.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://portlandpress.com/biochemj/article-pdf/240/3/913/588955/bj2400913.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        11,
        25
      ]
    ],
    "date-time": "2021-11-25T16:53:20Z",
    "timestamp": 1637859200000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://portlandpress.com/biochemj/article/240/3/913/22654/Comparison-of-human-stromelysin-and-collagenase-by"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1986,
        12,
        15
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          1986,
          12,
          15
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1042/bj2400913",
  "relation": {},
  "ISSN": [
    "0264-6021",
    "1470-8728"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1986,
        12,
        15
      ]
    ]
  }
}