DOI: 10.1042/bj2370105. Molecular cloning of two cysteine proteinases from paw-paw (<i>Carica papaya</i>)

Molecular cloning of two cysteine proteinases from paw-paw (Carica papaya)

10.1042/bj2370105

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

Two cDNA clones for plant cysteine proteinases have been isolated from a Carica papaya (paw-paw, papaya) leaf tissue cDNA library by using a mixture of 16 synthetic oligodeoxyribonucleotides as a hybridization probe. The inserted regions are 311 and 440 base-pairs in length and have the potential to encode a region corresponding to the C-terminal region of two proteins which are homologous with the known plant cysteine proteinases and the mammalian thiol cathepsins. One of the sequences shows a high (greater than 77%) homology with the plant cysteine proteinase papain, the other is closely related to papaya chymopapain. One sequence contains all, and the other most, of the 3′ untranslated region of the mRNA. The inserts were used as specific probes in Northern Blot analyses giving an estimated size for the two mRNA species of 1.45 kilobases.

Bibliography

McKee, R. A., Adams, S., Matthews, J. A., Smith, C. J., & Smith, H. (1986). Molecular cloning of two cysteine proteinases from paw-paw (Carica papaya). Biochemical Journal, 237(1), 105â110.

Authors 5

R A McKee (first)
S Adams (additional)
J A Matthews (additional)
C J Smith (additional)
H Smith (additional)

References 0 Referenced 12

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 4:54 p.m.)
Deposited	3 years, 9 months ago (Nov. 25, 2021, 11:39 a.m.)
Indexed	1 year, 11 months ago (Sept. 15, 2023, 9:34 p.m.)
Issued	39 years, 1 month ago (July 1, 1986)
Published	39 years, 1 month ago (July 1, 1986)
Published Print	39 years, 1 month ago (July 1, 1986)

Funders 0

None

BibTeX

@article{McKee_1986, title={Molecular cloning of two cysteine proteinases from paw-paw (Carica papaya)}, volume={237}, ISSN={1470-8728}, url={http://dx.doi.org/10.1042/bj2370105}, DOI={10.1042/bj2370105}, number={1}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={McKee, R A and Adams, S and Matthews, J A and Smith, C J and Smith, H}, year={1986}, month=jul, pages={105–110} }

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