DOI: 10.1042/bj2320435. Size of the inositol 1,4,5-trisphosphate-sensitive calcium pool in guinea-pig hepatocytes

Size of the inositol 1,4,5-trisphosphate-sensitive calcium pool in guinea-pig hepatocytes

10.1042/bj2320435

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

Permeabilized hepatocytes accumulated 45Ca2+ into a non-mitochondrial pool when provided with ATP. 45Ca2+ efflux from this pool was revealed by removal of ATP with glucose and hexokinase or by inhibiting uptake with NaVO3. The effect of inositol 1,4,5-trisphosphate (IP3) on 45Ca2+ efflux from the pool was investigated. IP3 (5 microM) evoked a rapid increase in the rate of 45Ca2+ efflux. Kinetic analysis of the effect of IP3 indicated the existence of two distinct Ca2+ fractions within the pool; only one, accounting for about one-third of the ATP-dependent Ca2+ content of the pool, was responsive to IP3. The effect of IP3 on 45Ca2+ efflux from the non-mitochondrial pool does not require ATP, a finding that is inconsistent with a previous suggestion that this effect may be mediated by protein phosphorylation.

Bibliography

Taylor, C. W., & Putney, J. W. (1985). Size of the inositol 1,4,5-trisphosphate-sensitive calcium pool in guinea-pig hepatocytes. Biochemical Journal, 232(2), 435â438.

Authors 2

C W Taylor (first)
J W Putney (additional)

References 0 Referenced 45

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 4:50 p.m.)
Deposited	3 years, 9 months ago (Nov. 25, 2021, 10:30 a.m.)
Indexed	1 year, 2 months ago (June 17, 2024, 4:35 p.m.)
Issued	39 years, 9 months ago (Dec. 1, 1985)
Published	39 years, 9 months ago (Dec. 1, 1985)
Published Print	39 years, 9 months ago (Dec. 1, 1985)

Funders 0

None

BibTeX

@article{Taylor_1985, title={Size of the inositol 1,4,5-trisphosphate-sensitive calcium pool in guinea-pig hepatocytes}, volume={232}, ISSN={1470-8728}, url={http://dx.doi.org/10.1042/bj2320435}, DOI={10.1042/bj2320435}, number={2}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={Taylor, C W and Putney, J W}, year={1985}, month=dec, pages={435–438} }

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