DOI: 10.1042/bj2240525. Photoaffinity labelling of mitochondrial NADH dehydrogenase with arylazidoamorphigenin, an analogue of rotenone

Photoaffinity labelling of mitochondrial NADH dehydrogenase with arylazidoamorphigenin, an analogue of rotenone

10.1042/bj2240525

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

A photoaffinity-labelling analogue of the respiratory inhibitor rotenone was synthesized from the naturally occurring rotenoid amorphigenin. The analogue inhibits NADH-ubiquinone oxidoreductase activity at concentrations comparable with those of rotenone. Photolysis of the radiolabelled analogue bound to isolated NADH-ubiquinone oxidoreductase resulted in preferential incorporation of radioactivity into a polypeptide of Mr 33 000, particularly at low concentrations of the inhibitor. Preparations of the enzyme differ in a parallel fashion in the content of this polypeptide, the degree of photolabelling by the analogue and their sensitivity to rotenone, providing further evidence that the 33 000-Mr protein forms part of the rotenone-binding site.

Bibliography

Earley, F. G. P., & Ragan, C. I. (1984). Photoaffinity labelling of mitochondrial NADH dehydrogenase with arylazidoamorphigenin, an analogue of rotenone. Biochemical Journal, 224(2), 525â534.

Authors 2

F G P Earley (first)
C I Ragan (additional)

References 0 Referenced 55

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 4:46 p.m.)
Deposited	3 years, 9 months ago (Nov. 25, 2021, 9:51 a.m.)
Indexed	2 months ago (June 27, 2025, 6:37 a.m.)
Issued	40 years, 8 months ago (Dec. 1, 1984)
Published	40 years, 8 months ago (Dec. 1, 1984)
Published Print	40 years, 8 months ago (Dec. 1, 1984)

Funders 0

None

BibTeX

@article{Earley_1984, title={Photoaffinity labelling of mitochondrial NADH dehydrogenase with arylazidoamorphigenin, an analogue of rotenone}, volume={224}, ISSN={1470-8728}, url={http://dx.doi.org/10.1042/bj2240525}, DOI={10.1042/bj2240525}, number={2}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={Earley, F G P and Ragan, C I}, year={1984}, month=dec, pages={525–534} }

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