DOI: 10.1042/bj20020503. Extensive temporally regulated reorganization of the lipid raft proteome following T-cell antigen receptor triggering

Extensive temporally regulated reorganization of the lipid raft proteome following T-cell antigen receptor triggering

10.1042/bj20020503

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

Signalling by immunoreceptors is orchestrated at specific plasma membrane microdomains, referred to as lipid rafts. Here we present a proteomics approach to the temporal analysis of protein association with lipid rafts following T-cell antigen receptor (TCR) triggering. We show that TCR engagement promotes the temporally regulated recruitment of proteins participating in the TCR signalling cascade to lipid rafts. Furthermore, TCR triggering results in profound modifications in the composition of lipid rafts involving a number of proteins associated either directly or indirectly with both plasma and intracellular membranes. Raft-associated proteins can be clustered according to their temporal profile of raft association. The data identify lipid rafts as highly dynamic structures and reveal a dramatic impact of surface TCR triggering not only on components of the TCR signalling machinery but also on proteins implicated in a number of diverse cellular processes.

Bibliography

BINI, L., PACINI, S., LIBERATORI, S., VALENSIN, S., PELLEGRINI, M., RAGGIASCHI, R., PALLINI, V., & BALDARI, C. T. (2003). Extensive temporally regulated reorganization of the lipid raft proteome following T-cell antigen receptor triggering. Biochemical Journal, 369(2), 301â309.

Authors 8

Luca BINI (first)
Sonia PACINI (additional)
Sabrina LIBERATORI (additional)
Silvia VALENSIN (additional)
Michela PELLEGRINI (additional)
Roberto RAGGIASCHI (additional)
Vitaliano PALLINI (additional)
Cosima T. BALDARI (additional)

References 0 Referenced 114

None

Dates

Type	When
Created	22 years, 6 months ago (Feb. 19, 2003, 4:39 a.m.)
Deposited	3 years, 9 months ago (Nov. 22, 2021, 6:59 a.m.)
Indexed	1 year ago (Aug. 6, 2024, 2:05 a.m.)
Issued	22 years, 7 months ago (Jan. 15, 2003)
Published	22 years, 7 months ago (Jan. 15, 2003)
Published Print	22 years, 7 months ago (Jan. 15, 2003)

Funders 0

None

BibTeX

@article{BINI_2003, title={Extensive temporally regulated reorganization of the lipid raft proteome following T-cell antigen receptor triggering}, volume={369}, ISSN={1470-8728}, url={http://dx.doi.org/10.1042/bj20020503}, DOI={10.1042/bj20020503}, number={2}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={BINI, Luca and PACINI, Sonia and LIBERATORI, Sabrina and VALENSIN, Silvia and PELLEGRINI, Michela and RAGGIASCHI, Roberto and PALLINI, Vitaliano and BALDARI, Cosima T.}, year={2003}, month=jan, pages={301–309} }

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