DOI: 10.1042/bj1960693. Fluorimetric study of the binding of protoporphyrin to haemopexin and albumin

Fluorimetric study of the binding of protoporphyrin to haemopexin and albumin

10.1042/bj1960693

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

Fluorescence spectra of protoporphyrin bound to its most affinitive site on human serum albumin, bound to human haemopexin and dissolved in human plasma reveal that, when present in plasma, at least 90% of this porphyrin is bound to albumin. Human serum albumin binds protoporphyrin with an affinity KA = 3 } 10(9)M-1 in phosphate-buffered saline. The affinity of haemopexin for protoporphyrin is 4 times smaller. From these data it is concluded that less than 1% of plasma protoporphyrin is bound to haemopexin. Implications of the data for protoporphyrin transport and clearance are discussed.

Bibliography

Lamola, A. A., Asher, I., Muller-Eberhard, U., & Poh-Fitzpatrick, M. (1981). Fluorimetric study of the binding of protoporphyrin to haemopexin and albumin. Biochemical Journal, 196(3), 693â698.

Authors 4

A A Lamola (first)
I Asher (additional)
U Muller-Eberhard (additional)
M Poh-Fitzpatrick (additional)

References 0 Referenced 55

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 4:28 p.m.)
Deposited	3 years, 9 months ago (Nov. 25, 2021, 10:39 a.m.)
Indexed	1 year, 8 months ago (Jan. 2, 2024, 12:09 p.m.)
Issued	44 years, 2 months ago (June 15, 1981)
Published	44 years, 2 months ago (June 15, 1981)
Published Print	44 years, 2 months ago (June 15, 1981)

Funders 0

None

BibTeX

@article{Lamola_1981, title={Fluorimetric study of the binding of protoporphyrin to haemopexin and albumin}, volume={196}, ISSN={0264-6021}, url={http://dx.doi.org/10.1042/bj1960693}, DOI={10.1042/bj1960693}, number={3}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={Lamola, A A and Asher, I and Muller-Eberhard, U and Poh-Fitzpatrick, M}, year={1981}, month=jun, pages={693–698} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        1,
        2
      ]
    ],
    "date-time": "2024-01-02T17:09:43Z",
    "timestamp": 1704215383407
  },
  "reference-count": 0,
  "publisher": "Portland Press Ltd.",
  "issue": "3",
  "content-domain": {
    "domain": [
      "portlandpress.com"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1981,
        6,
        15
      ]
    ]
  },
  "abstract": "<jats:p>Fluorescence spectra of protoporphyrin bound to its most affinitive site on human serum albumin, bound to human haemopexin and dissolved in human plasma reveal that, when present in plasma, at least 90% of this porphyrin is bound to albumin. Human serum albumin binds protoporphyrin with an affinity KA = 3 } 10(9)M-1 in phosphate-buffered saline. The affinity of haemopexin for protoporphyrin is 4 times smaller. From these data it is concluded that less than 1% of plasma protoporphyrin is bound to haemopexin. Implications of the data for protoporphyrin transport and clearance are discussed.</jats:p>",
  "DOI": "10.1042/bj1960693",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2015,
        8,
        10
      ]
    ],
    "date-time": "2015-08-10T20:28:08Z",
    "timestamp": 1439238488000
  },
  "page": "693-698",
  "update-policy": "http://dx.doi.org/10.1042/crossmark_policy",
  "source": "Crossref",
  "is-referenced-by-count": 55,
  "title": "Fluorimetric study of the binding of protoporphyrin to haemopexin and albumin",
  "prefix": "10.1042",
  "volume": "196",
  "author": [
    {
      "given": "A A",
      "family": "Lamola",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "I",
      "family": "Asher",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "U",
      "family": "Muller-Eberhard",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "M",
      "family": "Poh-Fitzpatrick",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "288",
  "container-title": "Biochemical Journal",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://portlandpress.com/biochemj/article-pdf/196/3/693/564883/bj1960693.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://portlandpress.com/biochemj/article-pdf/196/3/693/564883/bj1960693.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        11,
        25
      ]
    ],
    "date-time": "2021-11-25T15:39:41Z",
    "timestamp": 1637854781000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://portlandpress.com/biochemj/article/196/3/693/20914/Fluorimetric-study-of-the-binding-of"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1981,
        6,
        15
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          1981,
          6,
          15
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1042/bj1960693",
  "relation": {},
  "ISSN": [
    "0264-6021"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1981,
        6,
        15
      ]
    ]
  }
}