DOI: 10.1042/bj1950159. Purification of rabbit bone inhibitor of collagenase

Purification of rabbit bone inhibitor of collagenase

10.1042/bj1950159

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

1. Rabbit bones in tissue culture synthesize an inhibitor of collagenase during the first 4 days of culture. 2. The inhibitor was purified by a combination of gel filtration, concanavalin A--Sepharose chromatography, ion-exchange chromatography and zinc-chelate affinity chromatography. 3. The purified inhibitor migrated as a single band on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and had a mol.wt. of 28000. 4. The inhibitor blocked the activity of the metalloproteinases collagenase, gelatinase, neutral proteinase III (proteoglycanase), human leucocyte collagenase and gelatinase, but not thermolysin or bacterial collagenase. The serine proteinases plasmin and trypsin were not inhibited. 5. The inhibitor interacted with purified rabbit bone collagenase with 1:1 stoichiometry. 6. The inhibitory activity was lost after incubation for 1 h at 90 degrees C, after treatment with trypsin (250 micrograms/ml) at 37 degrees C for 30 min and after reduction and alkylation.

Bibliography

Cawston, T. E., Galloway, W. A., Mercer, E., Murphy, G., & Reynolds, J. J. (1981). Purification of rabbit bone inhibitor of collagenase. Biochemical Journal, 195(1), 159â165.

Authors 5

T E Cawston (first)
W A Galloway (additional)
E Mercer (additional)
G Murphy (additional)
J J Reynolds (additional)

References 0 Referenced 257

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 4:27 p.m.)
Deposited	3 years, 9 months ago (Nov. 25, 2021, 7:07 a.m.)
Indexed	2 days ago (Sept. 4, 2025, 10:07 a.m.)
Issued	44 years, 5 months ago (April 1, 1981)
Published	44 years, 5 months ago (April 1, 1981)
Published Print	44 years, 5 months ago (April 1, 1981)

Funders 0

None

BibTeX

@article{Cawston_1981, title={Purification of rabbit bone inhibitor of collagenase}, volume={195}, ISSN={0264-6021}, url={http://dx.doi.org/10.1042/bj1950159}, DOI={10.1042/bj1950159}, number={1}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={Cawston, T E and Galloway, W A and Mercer, E and Murphy, G and Reynolds, J J}, year={1981}, month=apr, pages={159–165} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        9,
        4
      ]
    ],
    "date-time": "2025-09-04T14:07:17Z",
    "timestamp": 1756994837946
  },
  "reference-count": 0,
  "publisher": "Portland Press Ltd.",
  "issue": "1",
  "content-domain": {
    "domain": [
      "portlandpress.com"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1981,
        4,
        1
      ]
    ]
  },
  "abstract": "<jats:p>1. Rabbit bones in tissue culture synthesize an inhibitor of collagenase during the first 4 days of culture. 2. The inhibitor was purified by a combination of gel filtration, concanavalin A--Sepharose chromatography, ion-exchange chromatography and zinc-chelate affinity chromatography. 3. The purified inhibitor migrated as a single band on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and had a mol.wt. of 28000. 4. The inhibitor blocked the activity of the metalloproteinases collagenase, gelatinase, neutral proteinase III (proteoglycanase), human leucocyte collagenase and gelatinase, but not thermolysin or bacterial collagenase. The serine proteinases plasmin and trypsin were not inhibited. 5. The inhibitor interacted with purified rabbit bone collagenase with 1:1 stoichiometry. 6. The inhibitory activity was lost after incubation for 1 h at 90 degrees C, after treatment with trypsin (250 micrograms/ml) at 37 degrees C for 30 min and after reduction and alkylation.</jats:p>",
  "DOI": "10.1042/bj1950159",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2015,
        8,
        10
      ]
    ],
    "date-time": "2015-08-10T20:27:43Z",
    "timestamp": 1439238463000
  },
  "page": "159-165",
  "update-policy": "http://dx.doi.org/10.1042/crossmark_policy",
  "source": "Crossref",
  "is-referenced-by-count": 257,
  "title": "Purification of rabbit bone inhibitor of collagenase",
  "prefix": "10.1042",
  "volume": "195",
  "author": [
    {
      "given": "T E",
      "family": "Cawston",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "W A",
      "family": "Galloway",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "E",
      "family": "Mercer",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "G",
      "family": "Murphy",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "J J",
      "family": "Reynolds",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "288",
  "container-title": "Biochemical Journal",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://portlandpress.com/biochemj/article-pdf/195/1/159/576349/bj1950159.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://portlandpress.com/biochemj/article-pdf/195/1/159/576349/bj1950159.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        11,
        25
      ]
    ],
    "date-time": "2021-11-25T12:07:29Z",
    "timestamp": 1637842049000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://portlandpress.com/biochemj/article/195/1/159/6359/Purification-of-rabbit-bone-inhibitor-of"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1981,
        4,
        1
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          1981,
          4,
          1
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1042/bj1950159",
  "relation": {},
  "ISSN": [
    "0264-6021"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1981,
        4,
        1
      ]
    ]
  }
}