DOI: 10.1042/bj1910095. Kinetics and mechanism of the interaction between human serum albumin and monomeric haemin

Kinetics and mechanism of the interaction between human serum albumin and monomeric haemin

10.1042/bj1910095

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

The interaction of human serum albumin with monomeric haemin has been investigated by detailed kinetic analysis in dimethyl sulphoxide/water (3:5, v/v). The results obtained under conditions of albumin saturation of haemin and under pseudo-single turnover conditions indicate that methaemalbumin is formed in a two-stage, single-intermediate process. The initial association between the haemin and human serum albumin is a chemically controlled process (k1 = 1.7 × 10(5) mol-1 . s-1 . dm3 at 24 degrees C); the variation of K1 with pH exhibited a well defined pK of 5.9. The overall equilibrium constant, calculated by using microscopic rate constants, is 1.1 (+/- 0.5) X 10(8) mol-1 at 24 degrees C. The data and conclusions are consistent with a general binding mechanism for albumin in which intermediate formation is followed by an entropy-controlled internalization of the ligand.

Bibliography

Adams, P. A., & Berman, M. C. (1980). Kinetics and mechanism of the interaction between human serum albumin and monomeric haemin. Biochemical Journal, 191(1), 95â102.

Authors 2

P A Adams (first)
M C Berman (additional)

References 0 Referenced 124

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 4:24 p.m.)
Deposited	3 years, 9 months ago (Nov. 25, 2021, 7:24 a.m.)
Indexed	11 months, 2 weeks ago (Sept. 13, 2024, 12:42 p.m.)
Issued	44 years, 10 months ago (Oct. 1, 1980)
Published	44 years, 10 months ago (Oct. 1, 1980)
Published Print	44 years, 10 months ago (Oct. 1, 1980)

Funders 0

None

BibTeX

@article{Adams_1980, title={Kinetics and mechanism of the interaction between human serum albumin and monomeric haemin}, volume={191}, ISSN={0264-6021}, url={http://dx.doi.org/10.1042/bj1910095}, DOI={10.1042/bj1910095}, number={1}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={Adams, P A and Berman, M C}, year={1980}, month=oct, pages={95–102} }

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