DOI: 10.1042/bj1880047. Reconstitution of the Ca2+-transport system of human erythrocytes

Reconstitution of the Ca2+-transport system of human erythrocytes

10.1042/bj1880047

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

The (Ca2+ + Mg2+)-dependent ATPase of human erythrocyte ‘ghosts’ was solubilized and reconstituted to form membranous vesicles capable of energized Ca2+ accumulation. The erythrocyte ‘ghosts’ for this purpose were prepared by using isoosmotic freeze-haemolysis in the presence of Tween 20 and proteinase inhibitors to stabilize the preparation. The reconstitution procedure is similar to that developed by Meissner & Fleischer [(1974) J. Biol. Chem. 249, 302-309] for skeletal-muscle sarcoplasmic-reticulum in that: (1) deoxycholate is used for the solubilization of the membrane; (2) controlled dialysis at near room temperature, rather than 0 degree C, is required in order to obtain a functional preparation capable of Ca2+ accumulation; and (3) membrane vesicles can be reassembled with protein/lipid ratio (approx. 60% protein and 40% lipid) similar to that of the original membrane.

Bibliography

Gietzen, K., Seiler, S., Fleischer, S., & Wolf, H. U. (1980). Reconstitution of the Ca2+-transport system of human erythrocytes. Biochemical Journal, 188(1), 47â54.

Authors 4

K Gietzen (first)
S Seiler (additional)
S Fleischer (additional)
H U Wolf (additional)

References 0 Referenced 22

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 4:22 p.m.)
Deposited	3 years, 8 months ago (Nov. 26, 2021, 8:57 a.m.)
Indexed	1 year, 9 months ago (Nov. 22, 2023, 10:43 p.m.)
Issued	45 years, 4 months ago (April 15, 1980)
Published	45 years, 4 months ago (April 15, 1980)
Published Print	45 years, 4 months ago (April 15, 1980)

Funders 0

None

BibTeX

@article{Gietzen_1980, title={Reconstitution of the Ca2+-transport system of human erythrocytes}, volume={188}, ISSN={0264-6021}, url={http://dx.doi.org/10.1042/bj1880047}, DOI={10.1042/bj1880047}, number={1}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={Gietzen, K and Seiler, S and Fleischer, S and Wolf, H U}, year={1980}, month=apr, pages={47–54} }

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