DOI: 10.1042/bj1760075. Properties of the membrane proteins of rat liver lysosomes. The majority of lysosomal membrane proteins are exposed to the cytoplasm

Properties of the membrane proteins of rat liver lysosomes. The majority of lysosomal membrane proteins are exposed to the cytoplasm

10.1042/bj1760075

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

Rat liver lysosomes were lysed and subfractionated by differential centrifugation through 0.2M-NaCl to yield a membranous pellet. This membrane fraction contains less than 20% of the lysosomal protein, adenosine triphosphatase activity of about 1.2mumol/min per mg of protein, 120nmol of thiol groups/mg of protein and at least 16 protein and glycoprotein bands on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. The gel patterns of membranes isolated from lysosomes after treatment with (1) [125I]iodidehydrogen peroxide-lactoperoxidase, (2) toluene 2,4-di-isocyanate-activated bovine serum albumin, (3) trypsin and (4) subtilisin indicate that most of the membrane proteins are exposed to the cytoplasm. These exposed proteins are candidates for intracellular receptors which recognize either substances that are to be degraded or vesicles containing those substances.

Bibliography

Schneider, D. L., Burnside, J., Gorga, F. R., & Nettleton, C. J. (1978). Properties of the membrane proteins of rat liver lysosomes. The majority of lysosomal membrane proteins are exposed to the cytoplasm. Biochemical Journal, 176(1), 75â82.

Authors 4

D L Schneider (first)
J Burnside (additional)
F R Gorga (additional)
C J Nettleton (additional)

References 0 Referenced 20

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 4:14 p.m.)
Deposited	3 years, 9 months ago (Nov. 26, 2021, 12:59 p.m.)
Indexed	3 years, 3 months ago (May 14, 2022, 2:24 p.m.)
Issued	46 years, 10 months ago (Oct. 15, 1978)
Published	46 years, 10 months ago (Oct. 15, 1978)
Published Print	46 years, 10 months ago (Oct. 15, 1978)

Funders 0

None

BibTeX

@article{Schneider_1978, title={Properties of the membrane proteins of rat liver lysosomes. The majority of lysosomal membrane proteins are exposed to the cytoplasm}, volume={176}, ISSN={0264-6021}, url={http://dx.doi.org/10.1042/bj1760075}, DOI={10.1042/bj1760075}, number={1}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={Schneider, D L and Burnside, J and Gorga, F R and Nettleton, C J}, year={1978}, month=oct, pages={75–82} }

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