DOI: 10.1042/bj1680239. The pinocytosis of 125I-labelled poly(vinylpyrrolidone), [14C]sucrose and colloidal [198Au]gold by rat yolk sac cultured <i>in vitro</i>

The pinocytosis of 125I-labelled poly(vinylpyrrolidone), [14C]sucrose and colloidal [198Au]gold by rat yolk sac cultured in vitro

10.1042/bj1680239

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

The rates of uptake of 125I-labelled poly(vinylpyrrolidone), [14C]sucrose and colloidal [198Au]gold by 17.5-day rat yolk sac cultured in vitro were studied. Over a 6.5h period each substrate was accumulated at a constant and reproducible rate of approx. 2microliter/h per mg of protein. After accumulation in vitro, the three substances were released from the tissue into substrate-free medium at low rates. Sucrose present in the medium at concentrations up to 10 mg/ml was without effect on the accumulation of either [14C]sucrose or 125I-labelled poly(vinylpyrrolidone), but at higher concentrations inhibited the uptake of both substrates. Some batches of colloidal [198Au]gold had a significantly higher Endocytic Index (up to 5 microliter/h per mg of protein). The Endocytic Index of such a batch decreased with increasing substrate concentration, but colloidal gold did not decrease the Endocytic Index of 125I-labelled poly(vinylpyrrolidone). It is concluded that the three substrates enter the yolk sac by pinocytosis in the liquid phase. Those batches of colloidal [198Au]gold with higher Endocytic Indices are considered to enter also by adsorption on membrane binding-sites.

Bibliography

Roberts, A. V. S., Williams, K. E., & Lloyd, J. B. (1977). The pinocytosis of 125I-labelled poly(vinylpyrrolidone), [14C]sucrose and colloidal [198Au]gold by rat yolk sac cultured in vitro. Biochemical Journal, 168(2), 239â244.

Authors 3

A V S Roberts (first)
K E Williams (additional)
J B Lloyd (additional)

References 0 Referenced 49

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 4:09 p.m.)
Deposited	3 years, 8 months ago (Nov. 26, 2021, 1:11 p.m.)
Indexed	1 year, 5 months ago (March 4, 2024, 10:24 a.m.)
Issued	47 years, 9 months ago (Nov. 15, 1977)
Published	47 years, 9 months ago (Nov. 15, 1977)
Published Print	47 years, 9 months ago (Nov. 15, 1977)

Funders 0

None

BibTeX

@article{Roberts_1977, title={The pinocytosis of 125I-labelled poly(vinylpyrrolidone), [14C]sucrose and colloidal [198Au]gold by rat yolk sac cultured in vitro}, volume={168}, ISSN={0306-3283}, url={http://dx.doi.org/10.1042/bj1680239}, DOI={10.1042/bj1680239}, number={2}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={Roberts, A V S and Williams, K E and Lloyd, J B}, year={1977}, month=nov, pages={239–244} }

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