DOI: 10.1042/bj1440319. Steady-state kinetics of catecholamine transport by chromaffin-granule ‘ghosts’

Steady-state kinetics of catecholamine transport by chromaffin-granule ‘ghosts’

10.1042/bj1440319

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

Resealed chromaffin-granule ‘ghosts’ were used to study the steady-state kinetics of catecholamine transport. The pump has a high affinity for (-)-noradrenaline, (-)-adrenaline, tyramine and 5-hydroxytryptamine (serotonin), but a lower affinity for (+)-noradrenaline. The measured rates of incorporation do not conform to Michaelis–Menten kinetics, but affinity constants for the former substrates are in the range 8–18μm. Reserpine is a potent inhibitor. Incorporation as a function of ATP concentration also fails to show simple kinetics; the affinity constant for ATP is deduced to be about 3mm at 1mm-MgCl2. Adenylyl (βγ-methylene)diphosphonate is a competitive inhibitor at low concentrations, but inhibits more strongly at high concentrations. The pump has a transition temperature at 29°C and does not seem to be identical with the Mg2+-stimulated adenosine triphosphatase of chromaffin granules.

Bibliography

Phillips, J. H. (1974). Steady-state kinetics of catecholamine transport by chromaffin-granule âghosts.â Biochemical Journal, 144(2), 319â325.

Authors 1

J H Phillips (first)

References 0 Referenced 52

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 3:56 p.m.)
Deposited	3 years, 9 months ago (Nov. 26, 2021, 11:16 a.m.)
Indexed	1 year, 9 months ago (Nov. 4, 2023, 4:21 p.m.)
Issued	50 years, 9 months ago (Nov. 15, 1974)
Published	50 years, 9 months ago (Nov. 15, 1974)
Published Print	50 years, 9 months ago (Nov. 15, 1974)

Funders 0

None

BibTeX

@article{Phillips_1974, title={Steady-state kinetics of catecholamine transport by chromaffin-granule ‘ghosts’}, volume={144}, ISSN={0306-3283}, url={http://dx.doi.org/10.1042/bj1440319}, DOI={10.1042/bj1440319}, number={2}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={Phillips, J H}, year={1974}, month=nov, pages={319–325} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2023,
        11,
        4
      ]
    ],
    "date-time": "2023-11-04T20:21:34Z",
    "timestamp": 1699129294679
  },
  "reference-count": 0,
  "publisher": "Portland Press Ltd.",
  "issue": "2",
  "content-domain": {
    "domain": [
      "portlandpress.com"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1974,
        11,
        15
      ]
    ]
  },
  "abstract": "<jats:p>Resealed chromaffin-granule \u2018ghosts\u2019 were used to study the steady-state kinetics of catecholamine transport. The pump has a high affinity for (-)-noradrenaline, (-)-adrenaline, tyramine and 5-hydroxytryptamine (serotonin), but a lower affinity for (+)-noradrenaline. The measured rates of incorporation do not conform to Michaelis\u2013Menten kinetics, but affinity constants for the former substrates are in the range 8\u201318\u03bcm. Reserpine is a potent inhibitor. Incorporation as a function of ATP concentration also fails to show simple kinetics; the affinity constant for ATP is deduced to be about 3mm at 1mm-MgCl2. Adenylyl (\u03b2\u03b3-methylene)diphosphonate is a competitive inhibitor at low concentrations, but inhibits more strongly at high concentrations. The pump has a transition temperature at 29\u00b0C and does not seem to be identical with the Mg2+-stimulated adenosine triphosphatase of chromaffin granules.</jats:p>",
  "DOI": "10.1042/bj1440319",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2015,
        8,
        10
      ]
    ],
    "date-time": "2015-08-10T19:56:16Z",
    "timestamp": 1439236576000
  },
  "page": "319-325",
  "update-policy": "http://dx.doi.org/10.1042/crossmark_policy",
  "source": "Crossref",
  "is-referenced-by-count": 52,
  "title": "Steady-state kinetics of catecholamine transport by chromaffin-granule \u2018ghosts\u2019",
  "prefix": "10.1042",
  "volume": "144",
  "author": [
    {
      "given": "J H",
      "family": "Phillips",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, U.K."
        }
      ]
    }
  ],
  "member": "288",
  "container-title": "Biochemical Journal",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://portlandpress.com/biochemj/article-pdf/144/2/319/781263/bj1440319.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://portlandpress.com/biochemj/article-pdf/144/2/319/781263/bj1440319.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        11,
        26
      ]
    ],
    "date-time": "2021-11-26T16:16:42Z",
    "timestamp": 1637943402000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://portlandpress.com/biochemj/article/144/2/319/9399/Steady-state-kinetics-of-catecholamine-transport"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1974,
        11,
        15
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "2",
    "published-print": {
      "date-parts": [
        [
          1974,
          11,
          15
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1042/bj1440319",
  "relation": {},
  "ISSN": [
    "0306-3283"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1974,
        11,
        15
      ]
    ]
  }
}