DOI: 10.1042/bj1400539. Compartmentation of free amino acids for protein synthesis in rat liver

Compartmentation of free amino acids for protein synthesis in rat liver

10.1042/bj1400539

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

The concept that a general intracellular pool serves as the sole precursor of amino acids for protein biosynthesis has been vigorously debated in recent years. To help resolve this controversy, we followed the distribution of intraperitoneally administered [3H]valine in the tRNA and the extracellular and intracellular compartments of rat liver. The specific radioactivity of the valine released from isolated tRNA was 2–3 times higher than that of intracellular valine, suggesting that the intracellular pool cannot be the sole precursor of amino acids used for charging tRNA. In addition, the specific radioactivity of the tRNA was only half that of the extracellular valine. Therefore it is unlikely that the valyl-tRNA is charged exclusively with amino acids derived from the extracellular pool. A model is proposed which stipulates that both extracellular and intracellular amino acids contribute to a restricted compartment that funnels amino acids towards protein biosynthesis.

Bibliography

Airhart, J., Vidrich, A., & Khairallah, E. A. (1974). Compartmentation of free amino acids for protein synthesis in rat liver. Biochemical Journal, 140(3), 539â545.

Authors 3

Judith Airhart (first)
Alda Vidrich (additional)
Edward A. Khairallah (additional)

References 0 Referenced 154

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 3:53 p.m.)
Deposited	3 years, 9 months ago (Nov. 26, 2021, 11:12 a.m.)
Indexed	11 months, 2 weeks ago (Sept. 12, 2024, 1:49 a.m.)
Issued	51 years, 2 months ago (June 1, 1974)
Published	51 years, 2 months ago (June 1, 1974)
Published Print	51 years, 2 months ago (June 1, 1974)

Funders 0

None

BibTeX

@article{Airhart_1974, title={Compartmentation of free amino acids for protein synthesis in rat liver}, volume={140}, ISSN={0264-6021}, url={http://dx.doi.org/10.1042/bj1400539}, DOI={10.1042/bj1400539}, number={3}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={Airhart, Judith and Vidrich, Alda and Khairallah, Edward A.}, year={1974}, month=jun, pages={539–545} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        9,
        12
      ]
    ],
    "date-time": "2024-09-12T05:49:36Z",
    "timestamp": 1726120176232
  },
  "reference-count": 0,
  "publisher": "Portland Press Ltd.",
  "issue": "3",
  "content-domain": {
    "domain": [
      "portlandpress.com"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1974,
        6,
        1
      ]
    ]
  },
  "abstract": "<jats:p>The concept that a general intracellular pool serves as the sole precursor of amino acids for protein biosynthesis has been vigorously debated in recent years. To help resolve this controversy, we followed the distribution of intraperitoneally administered [3H]valine in the tRNA and the extracellular and intracellular compartments of rat liver. The specific radioactivity of the valine released from isolated tRNA was 2\u20133 times higher than that of intracellular valine, suggesting that the intracellular pool cannot be the sole precursor of amino acids used for charging tRNA. In addition, the specific radioactivity of the tRNA was only half that of the extracellular valine. Therefore it is unlikely that the valyl-tRNA is charged exclusively with amino acids derived from the extracellular pool. A model is proposed which stipulates that both extracellular and intracellular amino acids contribute to a restricted compartment that funnels amino acids towards protein biosynthesis.</jats:p>",
  "DOI": "10.1042/bj1400539",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2015,
        8,
        10
      ]
    ],
    "date-time": "2015-08-10T19:53:43Z",
    "timestamp": 1439236423000
  },
  "page": "539-545",
  "update-policy": "http://dx.doi.org/10.1042/crossmark_policy",
  "source": "Crossref",
  "is-referenced-by-count": 154,
  "title": "Compartmentation of free amino acids for protein synthesis in rat liver",
  "prefix": "10.1042",
  "volume": "140",
  "author": [
    {
      "given": "Judith",
      "family": "Airhart",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Biochemistry and Biophysics, University of Connecticut, Storrs, Conn. 06268, U.S.A."
        }
      ]
    },
    {
      "given": "Alda",
      "family": "Vidrich",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Biochemistry and Biophysics, University of Connecticut, Storrs, Conn. 06268, U.S.A."
        }
      ]
    },
    {
      "given": "Edward A.",
      "family": "Khairallah",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Biochemistry and Biophysics, University of Connecticut, Storrs, Conn. 06268, U.S.A."
        }
      ]
    }
  ],
  "member": "288",
  "container-title": "Biochemical Journal",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://portlandpress.com/biochemj/article-pdf/140/3/539/562306/bj1400539.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://portlandpress.com/biochemj/article-pdf/140/3/539/562306/bj1400539.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        11,
        26
      ]
    ],
    "date-time": "2021-11-26T16:12:37Z",
    "timestamp": 1637943157000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://portlandpress.com/biochemj/article/140/3/539/9319/Compartmentation-of-free-amino-acids-for-protein"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1974,
        6,
        1
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          1974,
          6,
          1
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1042/bj1400539",
  "relation": {},
  "ISSN": [
    "0264-6021"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1974,
        6,
        1
      ]
    ]
  }
}