DOI: 10.1042/bj1390779. The use of radiolabelled T1 ribonuclease to monitor the efficiency of Edman degradations (<i>Short Communication</i>)

The use of radiolabelled T1 ribonuclease to monitor the efficiency of Edman degradations (Short Communication)

10.1042/bj1390779

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

A simple radiolabel tracer method is presented for monitoring the efficiency of the first ten cycles of an Edman degradation. This method consists of adding a very small amount of radiolabelled S-carboxymethylated T1 ribonuclease to the protein to be sequenced, and counting the radioactivity of a small sample from each of the degradative cycles.

Bibliography

McKean, D. J., & Smith, G. P. (1974). The use of radiolabelled T1 ribonuclease to monitor the efficiency of Edman degradations (Short Communication). Biochemical Journal, 139(3), 779â780.

Authors 2

David J. McKean (first)
George P. Smith (additional)

References 0 Referenced 5

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 3:53 p.m.)
Deposited	3 years, 9 months ago (Nov. 26, 2021, 10:59 a.m.)
Indexed	1 year, 11 months ago (Sept. 26, 2023, 9:33 a.m.)
Issued	51 years, 2 months ago (June 1, 1974)
Published	51 years, 2 months ago (June 1, 1974)
Published Print	51 years, 2 months ago (June 1, 1974)

Funders 0

None

BibTeX

@article{McKean_1974, title={The use of radiolabelled T1 ribonuclease to monitor the efficiency of Edman degradations (Short Communication)}, volume={139}, ISSN={0264-6021}, url={http://dx.doi.org/10.1042/bj1390779}, DOI={10.1042/bj1390779}, number={3}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={McKean, David J. and Smith, George P.}, year={1974}, month=jun, pages={779–780} }

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