DOI: 10.1042/bj1290197. The graphical determination of <i>Km</i> and <i>Ki</i>

The graphical determination of Km and Ki

10.1042/bj1290197

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

The principle of Dixon (1965) has been extended to give rapid graphical methods for determining enzyme constants for substrates (Km) and inhibitors (Ki). It does away with the sometimes questionable assumption that the amounts of substrate or inhibitor bound by the enzyme are negligible in comparison with the total amount added, and is therefore valid even for cases of high affinity, where the usual methods fail. Besides doing away with the need for calculation, it enables the concentrations of the various components of the system to be read off directly for any point of the velocity curve.

Bibliography

Dixon, M. (1972). The graphical determination of Km and Ki. Biochemical Journal, 129(1), 197â202.

Authors 1

M. Dixon (first)

References 0 Referenced 237

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 3:46 p.m.)
Deposited	3 years, 9 months ago (Nov. 26, 2021, 9:45 a.m.)
Indexed	1 month, 1 week ago (July 14, 2025, 11:49 p.m.)
Issued	53 years ago (Aug. 1, 1972)
Published	53 years ago (Aug. 1, 1972)
Published Print	53 years ago (Aug. 1, 1972)

Funders 0

None

BibTeX

@article{Dixon_1972, title={The graphical determination of Km and Ki}, volume={129}, ISSN={0306-3283}, url={http://dx.doi.org/10.1042/bj1290197}, DOI={10.1042/bj1290197}, number={1}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={Dixon, M.}, year={1972}, month=aug, pages={197–202} }

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