DOI: 10.1042/bj1250481. Ribonucleic acid–deoxyribonucleic acid hybridization in aqueous solutions and in solutions containing formamide

Ribonucleic acid–deoxyribonucleic acid hybridization in aqueous solutions and in solutions containing formamide

10.1042/bj1250481

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

Hybridization in 6×SSC (SSC, 0.15m-sodium chloride–0.015m-sodium citrate) at 66°C was compared with hybridization in formamide−6×SSC (1:1, v/v) at 35°C. As expected, the RNA hybridization potential was labile in the former system and stable in the latter. DNA retention by filters was poor in the formamide system, but could be improved. Several other properties of the hybridization reaction were explored and it was concluded that the formamide system is generally superior.

Bibliography

Gillespie, S., & Gillespie, D. (1971). Ribonucleic acidâdeoxyribonucleic acid hybridization in aqueous solutions and in solutions containing formamide. Biochemical Journal, 125(2), 481â487.

Authors 2

S. Gillespie (first)
D. Gillespie (additional)

References 0 Referenced 76

None

Dates

Type	When
Created	10 years ago (Aug. 13, 2015, 5:39 p.m.)
Deposited	3 years, 9 months ago (Nov. 26, 2021, 10:43 a.m.)
Indexed	4 months, 2 weeks ago (April 20, 2025, 12:01 a.m.)
Issued	53 years, 10 months ago (Nov. 1, 1971)
Published	53 years, 10 months ago (Nov. 1, 1971)
Published Print	53 years, 10 months ago (Nov. 1, 1971)

Funders 0

None

BibTeX

@article{Gillespie_1971, title={Ribonucleic acid–deoxyribonucleic acid hybridization in aqueous solutions and in solutions containing formamide}, volume={125}, ISSN={0306-3283}, url={http://dx.doi.org/10.1042/bj1250481}, DOI={10.1042/bj1250481}, number={2}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={Gillespie, S. and Gillespie, D.}, year={1971}, month=nov, pages={481–487} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        4,
        20
      ]
    ],
    "date-time": "2025-04-20T04:01:24Z",
    "timestamp": 1745121684358
  },
  "reference-count": 0,
  "publisher": "Portland Press Ltd.",
  "issue": "2",
  "content-domain": {
    "domain": [
      "portlandpress.com"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1971,
        11,
        1
      ]
    ]
  },
  "abstract": "<jats:p>Hybridization in 6\u00d7SSC (SSC, 0.15m-sodium chloride\u20130.015m-sodium citrate) at 66\u00b0C was compared with hybridization in formamide\u22126\u00d7SSC (1:1, v/v) at 35\u00b0C. As expected, the RNA hybridization potential was labile in the former system and stable in the latter. DNA retention by filters was poor in the formamide system, but could be improved. Several other properties of the hybridization reaction were explored and it was concluded that the formamide system is generally superior.</jats:p>",
  "DOI": "10.1042/bj1250481",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2015,
        8,
        13
      ]
    ],
    "date-time": "2015-08-13T21:39:23Z",
    "timestamp": 1439501963000
  },
  "page": "481-487",
  "update-policy": "http://dx.doi.org/10.1042/crossmark_policy",
  "source": "Crossref",
  "is-referenced-by-count": 76,
  "title": "Ribonucleic acid\u2013deoxyribonucleic acid hybridization in aqueous solutions and in solutions containing formamide",
  "prefix": "10.1042",
  "volume": "125",
  "author": [
    {
      "given": "S.",
      "family": "Gillespie",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Biology, Brandeis University, Waltham, Mass. 02154, U.S.A."
        }
      ]
    },
    {
      "given": "D.",
      "family": "Gillespie",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Biology, Brandeis University, Waltham, Mass. 02154, U.S.A."
        }
      ]
    }
  ],
  "member": "288",
  "container-title": "Biochemical Journal",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://portlandpress.com/biochemj/article-pdf/125/2/481/773892/bj1250481.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://portlandpress.com/biochemj/article-pdf/125/2/481/773892/bj1250481.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        11,
        26
      ]
    ],
    "date-time": "2021-11-26T15:43:21Z",
    "timestamp": 1637941401000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://portlandpress.com/biochemj/article/125/2/481/8463/Ribonucleic-acid-deoxyribonucleic-acid"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1971,
        11,
        1
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "2",
    "published-print": {
      "date-parts": [
        [
          1971,
          11,
          1
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1042/bj1250481",
  "relation": {},
  "ISSN": [
    "0306-3283"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1971,
        11,
        1
      ]
    ]
  }
}