DOI: 10.1042/bj1240921. Enzymic iodination. A probe for accessible surface proteins of normal and neoplastic lymphocytes

Enzymic iodination. A probe for accessible surface proteins of normal and neoplastic lymphocytes

10.1042/bj1240921

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

1. Radioactive iodide was covalently bound to living cells from normal mouse spleen and a variety of lymphoid tumours by a system consisting of lactoperoxidase, hydrogen peroxide and iodide. 2. About 3×105-6×105 molecules of [125I]iodide/cell could be incorporated without affecting cell viability. 3. Electron-micrographic radioautography showed that the radioactive label was associated with the outer surfaces of the cells. 4. Radioiodinated proteins were solubilized in 9m-urea–0.2m-mercaptoethanol and analysed by gel-filtration and disc electrophoresis. 5. Comparison of distinct tumour lines by disc electrophoresis showed qualitative and quantitative differences in protein distribution patterns.

Bibliography

Marchalonis, J. J., Cone, R. E., & Santer, V. (1971). Enzymic iodination. A probe for accessible surface proteins of normal and neoplastic lymphocytes. Biochemical Journal, 124(5), 921â927.

Authors 3

J J. Marchalonis (first)
R E. Cone (additional)
V Santer (additional)

References 0 Referenced 339

None

Dates

Type	When
Created	10 years ago (Aug. 13, 2015, 5:37 p.m.)
Deposited	3 years, 9 months ago (Nov. 26, 2021, 10:34 a.m.)
Indexed	2 days, 19 hours ago (Sept. 4, 2025, 9:30 a.m.)
Issued	53 years, 11 months ago (Oct. 1, 1971)
Published	53 years, 11 months ago (Oct. 1, 1971)
Published Print	53 years, 11 months ago (Oct. 1, 1971)

Funders 0

None

BibTeX

@article{Marchalonis_1971, title={Enzymic iodination. A probe for accessible surface proteins of normal and neoplastic lymphocytes}, volume={124}, ISSN={0306-3283}, url={http://dx.doi.org/10.1042/bj1240921}, DOI={10.1042/bj1240921}, number={5}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={Marchalonis, J J. and Cone, R E. and Santer, V}, year={1971}, month=oct, pages={921–927} }

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