DOI: 10.1042/bj1180253. Purification and properties of the nicotinamide–adenine dinucleotide phosphate-dependent isocitrate dehydrogenase from pig liver cytoplasm

Purification and properties of the nicotinamide–adenine dinucleotide phosphate-dependent isocitrate dehydrogenase from pig liver cytoplasm

10.1042/bj1180253

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

The NADP-dependent isocitrate dehydrogenase from pig liver soluble fraction was purified over 500-fold with an overall yield of 25%. The purified enzyme, which is homogeneous by all the usual criteria, has a molecular weight of about 75000 and is composed of two identical subunits. This has been demonstrated by ultracentrifugation, fluorescence titration and peptide `fingerprinting'. The maximal turnover number, extinction coefficients at 280nm and 260nm and amino acid analysis are described.

Bibliography

Illingworth, J. A., & Tipton, K. F. (1970). Purification and properties of the nicotinamideâadenine dinucleotide phosphate-dependent isocitrate dehydrogenase from pig liver cytoplasm. Biochemical Journal, 118(2), 253â258.

Authors 2

J. A. Illingworth (first)
K. F. Tipton (additional)

References 0 Referenced 40

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 3:28 p.m.)
Deposited	3 years, 9 months ago (Nov. 26, 2021, 2:38 p.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 5:41 p.m.)
Issued	55 years, 2 months ago (June 1, 1970)
Published	55 years, 2 months ago (June 1, 1970)
Published Print	55 years, 2 months ago (June 1, 1970)

Funders 0

None

BibTeX

@article{Illingworth_1970, title={Purification and properties of the nicotinamide–adenine dinucleotide phosphate-dependent isocitrate dehydrogenase from pig liver cytoplasm}, volume={118}, ISSN={0306-3283}, url={http://dx.doi.org/10.1042/bj1180253}, DOI={10.1042/bj1180253}, number={2}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={Illingworth, J. A. and Tipton, K. F.}, year={1970}, month=jun, pages={253–258} }

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