DOI: 10.1042/bj1060069. The purification and properties of two staphylolytic enzymes from <i>Streptomyces griseus</i>

The purification and properties of two staphylolytic enzymes from Streptomyces griseus

10.1042/bj1060069

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

1. Two staphylolytic enzymes have been purified from cultures of a soil isolate of Streptomyces griseus. 2. The purified enzymes were shown to be basic proteins of low molecular weight. Each enzyme released N-acetylmuramic acid reducing groups from the cell walls of Staphylococcus aureus. 3. The enzymes lysed whole staphylococci best at higher pH values and lower ionic strengths than when the substrate was isolated cell walls or purified mucopeptide. 4. Added teichoic acid did not inhibit the enzymes, but it formed an ethanol-precipitable complex with them. 5. The possibility that teichoic acid on the surface of whole cells prevents the access of the enzymes to their mucopeptide substrate is discussed.

Bibliography

Ward, J. B., & Perkins, H. R. (1968). The purification and properties of two staphylolytic enzymes from Streptomyces griseus. Biochemical Journal, 106(1), 69â76.

Authors 2

J. B. Ward (first)
H R Perkins (additional)

References 0 Referenced 10

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 3:13 p.m.)
Deposited	3 years, 8 months ago (Nov. 29, 2021, 3:48 p.m.)
Indexed	3 years, 4 months ago (April 3, 2022, 12:58 p.m.)
Issued	57 years, 7 months ago (Jan. 1, 1968)
Published	57 years, 7 months ago (Jan. 1, 1968)
Published Print	57 years, 7 months ago (Jan. 1, 1968)

Funders 0

None

BibTeX

@article{Ward_1968, title={The purification and properties of two staphylolytic enzymes from Streptomyces griseus}, volume={106}, ISSN={0306-3283}, url={http://dx.doi.org/10.1042/bj1060069}, DOI={10.1042/bj1060069}, number={1}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={Ward, J. B. and Perkins, H R}, year={1968}, month=jan, pages={69–76} }

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