DOI: 10.1042/bj1050279. Preparation and general properties of a soluble adenosine triphosphatase from mitochondria

Preparation and general properties of a soluble adenosine triphosphatase from mitochondria

10.1042/bj1050279

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

1. The purification of an adenosine triphosphatase present in aqueous extracts of acetone-dried ox-heart mitochondria is described. 2. No evidence was found for the presence of more than one protein having adenosine-triphosphatase activity in these extracts. 3. The enzyme is less stable at 0° than at 25° but is stabilized by glycerol. 4. The activity is dependent on the presence of Mg2+ or certain other bivalent metal cations. 5. The adenosine-triphosphatase activity of the Mg2+-activated enzyme is enhanced by 2,4-dinitrophenol. 6. The kinetics of Mg2+ activation indicate that the ATP–Mg2+ complex is the important substrate: free ATP and Mg2+ are inhibitory. 7. This preparation of mitochondrial adenosine triphosphatase has many properties in common with the adenosine triphosphatase coupling factor from mitochondria (Racker, 1961).

Bibliography

Selwyn, M. J. (1967). Preparation and general properties of a soluble adenosine triphosphatase from mitochondria. Biochemical Journal, 105(1), 279â288.

Authors 1

M J Selwyn (first)

References 0 Referenced 63

None

Dates

Type	When
Created	10 years ago (Aug. 13, 2015, 5:36 p.m.)
Deposited	3 years, 8 months ago (Nov. 29, 2021, 3:37 p.m.)
Indexed	1 year, 8 months ago (Dec. 11, 2023, 1:58 a.m.)
Issued	57 years, 10 months ago (Oct. 1, 1967)
Published	57 years, 10 months ago (Oct. 1, 1967)
Published Print	57 years, 10 months ago (Oct. 1, 1967)

Funders 0

None

BibTeX

@article{Selwyn_1967, title={Preparation and general properties of a soluble adenosine triphosphatase from mitochondria}, volume={105}, ISSN={0306-3283}, url={http://dx.doi.org/10.1042/bj1050279}, DOI={10.1042/bj1050279}, number={1}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={Selwyn, M J}, year={1967}, month=oct, pages={279–288} }

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