DOI: 10.1042/bst20170351. Fine details in complex environments: the power of cryo-electron tomography

Fine details in complex environments: the power of cryo-electron tomography

10.1042/bst20170351

Crossref journal-article

Portland Press Ltd.

Biochemical Society Transactions (288)

Abstract

Cryo-electron tomography (CET) is uniquely suited to obtain structural information from a wide range of biological scales, integrating and bridging knowledge from molecules to cells. In particular, CET can be used to visualise molecular structures in their native environment. Depending on the experiment, a varying degree of resolutions can be achieved, with the first near-atomic molecular structures becoming recently available. The power of CET has increased significantly in the last 5 years, in parallel with improvements in cryo-EM hardware and software that have also benefited single-particle reconstruction techniques. In this review, we cover the typical CET pipeline, starting from sample preparation, to data collection and processing, and highlight in particular the recent developments that support structural biology in situ. We provide some examples that highlight the importance of structure determination of molecules embedded within their native environment, and propose future directions to improve CET performance and accessibility.

Bibliography

Hutchings, J., & Zanetti, G. (2018). Fine details in complex environments: the power of cryo-electron tomography. Biochemical Society Transactions, 46(4), 807â816.

Authors 2

Joshua Hutchings (first)
Giulia Zanetti (additional)

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Dates

Type	When
Created	7 years, 2 months ago (June 22, 2018, 12:55 p.m.)
Deposited	3 years, 9 months ago (Nov. 18, 2021, 1:20 p.m.)
Indexed	9 months ago (Nov. 19, 2024, 12:35 p.m.)
Issued	7 years, 2 months ago (June 22, 2018)
Published	7 years, 2 months ago (June 22, 2018)
Published Online	7 years, 2 months ago (June 22, 2018)
Published Print	7 years ago (Aug. 20, 2018)

Funders 0

None

BibTeX

@article{Hutchings_2018, title={Fine details in complex environments: the power of cryo-electron tomography}, volume={46}, ISSN={1470-8752}, url={http://dx.doi.org/10.1042/bst20170351}, DOI={10.1042/bst20170351}, number={4}, journal={Biochemical Society Transactions}, publisher={Portland Press Ltd.}, author={Hutchings, Joshua and Zanetti, Giulia}, year={2018}, month=jun, pages={807–816} }

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