DOI: 10.1042/bst0340356. Sterol trafficking between the endoplasmic reticulum and plasma membrane in yeast

Sterol trafficking between the endoplasmic reticulum and plasma membrane in yeast

10.1042/bst0340356

Crossref journal-article

Portland Press Ltd.

Biochemical Society Transactions (288)

Abstract

We recently showed that transport of ergosterol from the ER (endoplasmic reticulum) to the sterol-enriched PM (plasma membrane) in yeast occurs by a non-vesicular (Sec18p-independent) mechanism that results in the equilibration of sterol pools in the two organelles [Baumann, Sullivan, Ohvo-Rekilä, Simonot, Pottekat, Klaassen, Beh and Menon (2005) Biochemistry 44, 5816–5826]. To explore how this occurs, we tested the role of proteins that might act as sterol transporters. We chose to study oxysterol-binding protein homologues (Osh proteins), a family of seven proteins in yeast, all of which contain a putative sterol-binding pocket. Recent structural analyses of one of the Osh proteins [Im, Raychaudhuri, Prinz and Hurley (2005) Nature (London) 437, 154–158] suggested a possible transport cycle in which Osh proteins could act to equilibrate ER and PM pools of sterol. Our results indicate that the transport of newly synthesized ergosterol from the ER to the PM in an OSH deletion mutant lacking all seven Osh proteins is slowed only 5-fold relative to the isogenic wild-type strain. Our results suggest that the Osh proteins are not sterol transporters themselves, but affect sterol transport in vivo indirectly by affecting the ability of the PM to sequester sterols.

Bibliography

Sullivan, D. P., Ohvo-RekilÃ¤, H., Baumann, N. A., Beh, C. T., & Menon, A. K. (2006). Sterol trafficking between the endoplasmic reticulum and plasma membrane in yeast. Biochemical Society Transactions, 34(3), 356â358.

Authors 5

D.P. Sullivan (first)
H. Ohvo-Rekilä (additional)
N.A. Baumann (additional)
C.T. Beh (additional)
A.K. Menon (additional)

References 25 Referenced 75

10.1016/j.ceb.2004.06.004 / Curr. Opin. Cell Biol. by van Meer (2004)
10.1038/nrm1591 / Nat. Rev. Mol. Cell Biol. by Holthuis (2005)
10.1038/sj.emboj.7600798 / EMBO J. by van Meer (2005)
10.1016/j.ceb.2005.06.005 / Curr. Opin. Cell Biol. by Levine (2005)
10.1042/BST0340335 / Biochem. Soc. Trans. by Maxfield (2006)
10.1146/annurev.biophys.32.110601.141704 / Annu. Rev. Biophys. Biomol. Struct. by McConnell (2003)
10.1146/annurev.cellbio.14.1.111 / Annu. Rev. Cell Dev. Biol. by Brown (1998)
10.1016/S0021-9258(19)39918-1 / J. Biol. Chem. by Urbani (1990)
10.1073/pnas.140218797 / Proc. Natl. Acad. Sci. U.S.A. by Heino (2000)
10.1021/bi048296z / Biochemistry by Baumann (2005)
10.1083/jcb.114.2.207 / J. Cell Biol. by Graham (1991)
10.1016/j.bbalip.2004.11.016 / Biochim. Biophys. Acta by Schnabl (2005)
10.1016/S0021-9258(18)32920-X / J. Biol. Chem. by Wattenberg (1983)
10.1016/S0021-9258(18)37666-X / J. Biol. Chem. by Steck (1988)
10.1074/jbc.M407600200 / J. Biol. Chem. by Li (2004)
10.1083/jcb.200503033 / J. Cell Biol. by Jüschke (2005)
10.1042/BST0331186 / Biochem. Soc. Trans. by Reiner (2005)
10.1038/nature02188 / Nature (London) by Hanada (2003)
10.1093/genetics/157.3.1117 / Genetics by Beh (2001)
10.1242/jcs.01157 / J. Cell Sci. by Beh (2004)
10.1038/nature03923 / Nature (London) by Im (2005)
10.1002/(SICI)1097-0061(199905)15:7<601::AID-YEA390>3.0.CO;2-N / Yeast by Daum (1999)
10.1016/0076-6879(91)94004-V / Methods Enzymol. by Sherman (1991)
10.1016/S0006-3495(00)76551-9 / Biophys. J. by Petrache (2000)
10.1007/978-1-4757-2065-5 / Biomembranes: Molecular Structure and Function by Gennis (1989)

Dates

Type	When
Created	19 years, 3 months ago (May 22, 2006, 7:52 a.m.)
Deposited	3 years, 9 months ago (Nov. 17, 2021, 3:31 p.m.)
Indexed	1 week, 1 day ago (Aug. 23, 2025, 1:02 a.m.)
Issued	19 years, 3 months ago (May 22, 2006)
Published	19 years, 3 months ago (May 22, 2006)
Published Online	19 years, 3 months ago (May 22, 2006)
Published Print	19 years, 2 months ago (June 1, 2006)

Funders 0

None

BibTeX

@article{Sullivan_2006, title={Sterol trafficking between the endoplasmic reticulum and plasma membrane in yeast}, volume={34}, ISSN={1470-8752}, url={http://dx.doi.org/10.1042/bst0340356}, DOI={10.1042/bst0340356}, number={3}, journal={Biochemical Society Transactions}, publisher={Portland Press Ltd.}, author={Sullivan, D.P. and Ohvo-Rekilä, H. and Baumann, N.A. and Beh, C.T. and Menon, A.K.}, year={2006}, month=may, pages={356–358} }

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