DOI: 10.1042/bst0330639. Localization and function of Arf family GTPases

Localization and function of Arf family GTPases

10.1042/bst0330639

Crossref journal-article

Portland Press Ltd.

Biochemical Society Transactions (288)

Abstract

Arfs are a family of Ras-related GTP-binding proteins that function in the regulation of membrane trafficking and structure. The six mammalian Arf proteins are expressed ubiquitously and so it is anticipated that each will have a distinct localization and function within the cell. It has been assumed that much of this specificity will be defined by determining which regulators of Arfs, the GEFs (guanine nucleotide-exchange factors) and GAPs (GTPase-activating proteins) function with which Arf proteins. Although in vitro assays may indicate Arf preferences for the numerous Arf GEFs and GAPs that have been identified, in the cell the different Arfs, GEFs and GAPs are targeted to specific compartments where they carry out their functions. We have embarked on studies to define regions of the Arf1 and Arf6 proteins that determine their sites of action and specific activities at the Golgi and plasma membrane respectively. Chimaeras were made between Arf1 and Arf6 in order to identify regions of the protein that contributed to targeting and function. Whereas Arf6 is targeted to the plasma membrane through multiple regions along the protein, we have found a Golgi-targeting region in Arf1 that is sufficient to target Arf6 to the Golgi complex.

Bibliography

Donaldson, J. G., & Honda, A. (2005). Localization and function of Arf family GTPases. Biochemical Society Transactions, 33(4), 639â642.

Authors 2

J.G. Donaldson (first)
A. Honda (additional)

References 34 Referenced 142

10.1016/S0955-0674(99)80067-2 / Curr. Opin. Cell Biol. by Chavrier (1999)
10.1016/S0955-0674(03)00071-1 / Curr. Opin. Cell Biol. by Nie (2003)
10.1016/S0962-8924(99)01699-2 / Trends Cell Biol. by Jackson (2000)
10.1016/j.cellsig.2003.09.012 / Cell. Signal. by Randazzo (2004)
10.1016/S0092-8674(03)01079-1 / Cell (Cambridge, Mass.) by Bonifacino (2004)
10.1016/j.tcb.2004.02.002 / Trends Cell Biol. by Robinson (2004)
10.1016/0092-8674(93)90323-I / Cell (Cambridge, Mass.) by Brown (1993)
10.1126/science.8290961 / Science by Cockcroft (1994)
10.1016/S0092-8674(00)81540-8 / Cell (Cambridge, Mass.) by Honda (1999)
10.1074/jbc.272.7.4141 / J. Biol. Chem. by Liang (1997)
10.1006/bbrc.2000.2700 / Biochem. Biophys. Res. Commun. by Takatsu (2000)
10.1074/jbc.272.52.33001 / J. Biol. Chem. by Liang (1997)
{'key': '2021111719401809300_B13', 'first-page': '71', 'volume': '146', 'author': 'Claude', 'year': '1999', 'journal-title': 'J. Cell Biol.'} / J. Cell Biol. by Claude (1999)
10.1034/j.1600-0854.2002.30705.x / Traffic by Kawamoto (2002)
10.1042/bj20020428 / Biochem. J. by Takatsu (2002)
10.1083/jcb.128.6.1003 / J. Cell Biol. by Peters (1995)
10.1074/jbc.R300026200 / J. Biol. Chem. by Donaldson (2003)
10.1083/jcb.200103107 / J. Cell Biol. by Brown (2001)
10.1073/pnas.2232129100 / Proc. Natl. Acad. Sci. U.S.A. by Lawrence (2003)
10.1146/annurev.physiol.65.092101.142517 / Annu. Rev. Physiol. by Yin (2003)
10.1083/jcb.200104019 / J. Cell Biol. by Santy (2001)
10.1111/j.1749-6632.2002.tb04463.x / Ann. N. Y. Acad. Sci. by Vitale (2002)
10.1083/jcb.200301006 / J. Cell Biol. by Krauss (2003)
10.1016/S0092-8674(00)81754-7 / Cell (Cambridge, Mass.) by Goldberg (1998)
10.1093/embo-reports/kve043 / EMBO Rep. by Pasqualato (2001)
10.1128/MCB.20.16.5998-6007.2000 / Mol. Cell. Biol. by Al-Awar (2000)
10.1083/jcb.200409138 / J. Cell Biol. by Honda (2005)
10.1083/jcb.200112092 / J. Cell Biol. by Rein (2002)
10.1083/jcb.141.7.1489 / J. Cell Biol. by Hay (1998)
10.1074/jbc.M007684200 / J. Biol. Chem. by Xu (2000)
10.1083/jcb.200408135 / J. Cell Biol. by Xu (2004)
10.1038/sj.embor.embor762 / EMBO Rep. by Garcia-Mata (2003)
10.1083/jcb.200404008 / J. Cell Biol. by Lee (2005)
10.1074/jbc.M112427200 / J. Biol. Chem. by Shinotsuka (2002)

Dates

Type	When
Created	20 years ago (Aug. 3, 2005, 8:51 a.m.)
Deposited	3 years, 9 months ago (Nov. 17, 2021, 5 p.m.)
Indexed	3 weeks, 3 days ago (Aug. 2, 2025, 12:02 a.m.)
Issued	20 years ago (Aug. 1, 2005)
Published	20 years ago (Aug. 1, 2005)
Published Online	20 years ago (Aug. 1, 2005)
Published Print	20 years ago (Aug. 1, 2005)

Funders 0

None

BibTeX

@article{Donaldson_2005, title={Localization and function of Arf family GTPases}, volume={33}, ISSN={1470-8752}, url={http://dx.doi.org/10.1042/bst0330639}, DOI={10.1042/bst0330639}, number={4}, journal={Biochemical Society Transactions}, publisher={Portland Press Ltd.}, author={Donaldson, J.G. and Honda, A.}, year={2005}, month=aug, pages={639–642} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        2
      ]
    ],
    "date-time": "2025-08-02T04:02:25Z",
    "timestamp": 1754107345166
  },
  "reference-count": 34,
  "publisher": "Portland Press Ltd.",
  "issue": "4",
  "content-domain": {
    "domain": [
      "portlandpress.com"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        2005,
        8,
        1
      ]
    ]
  },
  "abstract": "<jats:p>Arfs are a family of Ras-related GTP-binding proteins that function in the regulation of membrane trafficking and structure. The six mammalian Arf proteins are expressed ubiquitously and so it is anticipated that each will have a distinct localization and function within the cell. It has been assumed that much of this specificity will be defined by determining which regulators of Arfs, the GEFs (guanine nucleotide-exchange factors) and GAPs (GTPase-activating proteins) function with which Arf proteins. Although in vitro assays may indicate Arf preferences for the numerous Arf GEFs and GAPs that have been identified, in the cell the different Arfs, GEFs and GAPs are targeted to specific compartments where they carry out their functions. We have embarked on studies to define regions of the Arf1 and Arf6 proteins that determine their sites of action and specific activities at the Golgi and plasma membrane respectively. Chimaeras were made between Arf1 and Arf6 in order to identify regions of the protein that contributed to targeting and function. Whereas Arf6 is targeted to the plasma membrane through multiple regions along the protein, we have found a Golgi-targeting region in Arf1 that is sufficient to target Arf6 to the Golgi complex.</jats:p>",
  "DOI": "10.1042/bst0330639",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        8,
        3
      ]
    ],
    "date-time": "2005-08-03T12:51:58Z",
    "timestamp": 1123073518000
  },
  "page": "639-642",
  "update-policy": "http://dx.doi.org/10.1042/crossmark_policy",
  "source": "Crossref",
  "is-referenced-by-count": 142,
  "title": "Localization and function of Arf family GTPases",
  "prefix": "10.1042",
  "volume": "33",
  "author": [
    {
      "given": "J.G.",
      "family": "Donaldson",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, U.S.A."
        }
      ]
    },
    {
      "given": "A.",
      "family": "Honda",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, U.S.A."
        }
      ]
    }
  ],
  "member": "288",
  "published-online": {
    "date-parts": [
      [
        2005,
        8,
        1
      ]
    ]
  },
  "reference": [
    {
      "key": "2021111719401809300_B1",
      "doi-asserted-by": "crossref",
      "first-page": "466",
      "DOI": "10.1016/S0955-0674(99)80067-2",
      "volume": "11",
      "author": "Chavrier",
      "year": "1999",
      "journal-title": "Curr. Opin. Cell Biol."
    },
    {
      "key": "2021111719401809300_B2",
      "doi-asserted-by": "crossref",
      "first-page": "396",
      "DOI": "10.1016/S0955-0674(03)00071-1",
      "volume": "15",
      "author": "Nie",
      "year": "2003",
      "journal-title": "Curr. Opin. Cell Biol."
    },
    {
      "key": "2021111719401809300_B3",
      "doi-asserted-by": "crossref",
      "first-page": "60",
      "DOI": "10.1016/S0962-8924(99)01699-2",
      "volume": "10",
      "author": "Jackson",
      "year": "2000",
      "journal-title": "Trends Cell Biol."
    },
    {
      "key": "2021111719401809300_B4",
      "doi-asserted-by": "crossref",
      "first-page": "401",
      "DOI": "10.1016/j.cellsig.2003.09.012",
      "volume": "16",
      "author": "Randazzo",
      "year": "2004",
      "journal-title": "Cell. Signal."
    },
    {
      "key": "2021111719401809300_B5",
      "doi-asserted-by": "crossref",
      "first-page": "153",
      "DOI": "10.1016/S0092-8674(03)01079-1",
      "volume": "116",
      "author": "Bonifacino",
      "year": "2004",
      "journal-title": "Cell (Cambridge, Mass.)"
    },
    {
      "key": "2021111719401809300_B6",
      "doi-asserted-by": "crossref",
      "first-page": "167",
      "DOI": "10.1016/j.tcb.2004.02.002",
      "volume": "14",
      "author": "Robinson",
      "year": "2004",
      "journal-title": "Trends Cell Biol."
    },
    {
      "key": "2021111719401809300_B7",
      "doi-asserted-by": "crossref",
      "first-page": "1137",
      "DOI": "10.1016/0092-8674(93)90323-I",
      "volume": "75",
      "author": "Brown",
      "year": "1993",
      "journal-title": "Cell (Cambridge, Mass.)"
    },
    {
      "key": "2021111719401809300_B8",
      "doi-asserted-by": "crossref",
      "first-page": "523",
      "DOI": "10.1126/science.8290961",
      "volume": "263",
      "author": "Cockcroft",
      "year": "1994",
      "journal-title": "Science"
    },
    {
      "key": "2021111719401809300_B9",
      "doi-asserted-by": "crossref",
      "first-page": "521",
      "DOI": "10.1016/S0092-8674(00)81540-8",
      "volume": "99",
      "author": "Honda",
      "year": "1999",
      "journal-title": "Cell (Cambridge, Mass.)"
    },
    {
      "key": "2021111719401809300_B10",
      "doi-asserted-by": "crossref",
      "first-page": "4141",
      "DOI": "10.1074/jbc.272.7.4141",
      "volume": "272",
      "author": "Liang",
      "year": "1997",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2021111719401809300_B11",
      "doi-asserted-by": "crossref",
      "first-page": "719",
      "DOI": "10.1006/bbrc.2000.2700",
      "volume": "271",
      "author": "Takatsu",
      "year": "2000",
      "journal-title": "Biochem. Biophys. Res. Commun."
    },
    {
      "key": "2021111719401809300_B12",
      "doi-asserted-by": "crossref",
      "first-page": "33001",
      "DOI": "10.1074/jbc.272.52.33001",
      "volume": "272",
      "author": "Liang",
      "year": "1997",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2021111719401809300_B13",
      "first-page": "71",
      "volume": "146",
      "author": "Claude",
      "year": "1999",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2021111719401809300_B14",
      "doi-asserted-by": "crossref",
      "first-page": "483",
      "DOI": "10.1034/j.1600-0854.2002.30705.x",
      "volume": "3",
      "author": "Kawamoto",
      "year": "2002",
      "journal-title": "Traffic"
    },
    {
      "key": "2021111719401809300_B15",
      "doi-asserted-by": "crossref",
      "first-page": "369",
      "DOI": "10.1042/bj20020428",
      "volume": "365",
      "author": "Takatsu",
      "year": "2002",
      "journal-title": "Biochem. J."
    },
    {
      "key": "2021111719401809300_B16",
      "doi-asserted-by": "crossref",
      "first-page": "1003",
      "DOI": "10.1083/jcb.128.6.1003",
      "volume": "128",
      "author": "Peters",
      "year": "1995",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2021111719401809300_B17",
      "doi-asserted-by": "crossref",
      "first-page": "41573",
      "DOI": "10.1074/jbc.R300026200",
      "volume": "278",
      "author": "Donaldson",
      "year": "2003",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2021111719401809300_B18",
      "doi-asserted-by": "crossref",
      "first-page": "1007",
      "DOI": "10.1083/jcb.200103107",
      "volume": "154",
      "author": "Brown",
      "year": "2001",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2021111719401809300_B19",
      "doi-asserted-by": "crossref",
      "first-page": "13320",
      "DOI": "10.1073/pnas.2232129100",
      "volume": "100",
      "author": "Lawrence",
      "year": "2003",
      "journal-title": "Proc. Natl. Acad. Sci. U.S.A."
    },
    {
      "key": "2021111719401809300_B20",
      "doi-asserted-by": "crossref",
      "first-page": "761",
      "DOI": "10.1146/annurev.physiol.65.092101.142517",
      "volume": "65",
      "author": "Yin",
      "year": "2003",
      "journal-title": "Annu. Rev. Physiol."
    },
    {
      "key": "2021111719401809300_B21",
      "doi-asserted-by": "crossref",
      "first-page": "599",
      "DOI": "10.1083/jcb.200104019",
      "volume": "154",
      "author": "Santy",
      "year": "2001",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2021111719401809300_B22",
      "doi-asserted-by": "crossref",
      "first-page": "193",
      "DOI": "10.1111/j.1749-6632.2002.tb04463.x",
      "volume": "971",
      "author": "Vitale",
      "year": "2002",
      "journal-title": "Ann. N. Y. Acad. Sci."
    },
    {
      "key": "2021111719401809300_B23",
      "doi-asserted-by": "crossref",
      "first-page": "113",
      "DOI": "10.1083/jcb.200301006",
      "volume": "162",
      "author": "Krauss",
      "year": "2003",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2021111719401809300_B24",
      "doi-asserted-by": "crossref",
      "first-page": "237",
      "DOI": "10.1016/S0092-8674(00)81754-7",
      "volume": "95",
      "author": "Goldberg",
      "year": "1998",
      "journal-title": "Cell (Cambridge, Mass.)"
    },
    {
      "key": "2021111719401809300_B25",
      "doi-asserted-by": "crossref",
      "first-page": "234",
      "DOI": "10.1093/embo-reports/kve043",
      "volume": "2",
      "author": "Pasqualato",
      "year": "2001",
      "journal-title": "EMBO Rep."
    },
    {
      "key": "2021111719401809300_B26",
      "doi-asserted-by": "crossref",
      "first-page": "5998",
      "DOI": "10.1128/MCB.20.16.5998-6007.2000",
      "volume": "20",
      "author": "Al-Awar",
      "year": "2000",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "2021111719401809300_B27",
      "doi-asserted-by": "crossref",
      "first-page": "1039",
      "DOI": "10.1083/jcb.200409138",
      "volume": "168",
      "author": "Honda",
      "year": "2005",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2021111719401809300_B28",
      "doi-asserted-by": "crossref",
      "first-page": "395",
      "DOI": "10.1083/jcb.200112092",
      "volume": "157",
      "author": "Rein",
      "year": "2002",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2021111719401809300_B29",
      "doi-asserted-by": "crossref",
      "first-page": "1489",
      "DOI": "10.1083/jcb.141.7.1489",
      "volume": "141",
      "author": "Hay",
      "year": "1998",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2021111719401809300_B30",
      "doi-asserted-by": "crossref",
      "first-page": "39631",
      "DOI": "10.1074/jbc.M007684200",
      "volume": "275",
      "author": "Xu",
      "year": "2000",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2021111719401809300_B31",
      "doi-asserted-by": "crossref",
      "first-page": "997",
      "DOI": "10.1083/jcb.200408135",
      "volume": "167",
      "author": "Xu",
      "year": "2004",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2021111719401809300_B32",
      "doi-asserted-by": "crossref",
      "first-page": "320",
      "DOI": "10.1038/sj.embor.embor762",
      "volume": "4",
      "author": "Garcia-Mata",
      "year": "2003",
      "journal-title": "EMBO Rep."
    },
    {
      "key": "2021111719401809300_B33",
      "doi-asserted-by": "crossref",
      "first-page": "281",
      "DOI": "10.1083/jcb.200404008",
      "volume": "168",
      "author": "Lee",
      "year": "2005",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2021111719401809300_B34",
      "doi-asserted-by": "crossref",
      "first-page": "9468",
      "DOI": "10.1074/jbc.M112427200",
      "volume": "277",
      "author": "Shinotsuka",
      "year": "2002",
      "journal-title": "J. Biol. Chem."
    }
  ],
  "container-title": "Biochemical Society Transactions",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://portlandpress.com/biochemsoctrans/article-pdf/33/4/639/540479/bst0330639.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://portlandpress.com/biochemsoctrans/article-pdf/33/4/639/540479/bst0330639.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        11,
        17
      ]
    ],
    "date-time": "2021-11-17T22:00:47Z",
    "timestamp": 1637186447000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://portlandpress.com/biochemsoctrans/article/33/4/639/65145/Localization-and-function-of-Arf-family-GTPases"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2005,
        8,
        1
      ]
    ]
  },
  "references-count": 34,
  "journal-issue": {
    "issue": "4",
    "published-online": {
      "date-parts": [
        [
          2005,
          8,
          1
        ]
      ]
    },
    "published-print": {
      "date-parts": [
        [
          2005,
          8,
          1
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1042/BST0330639",
  "relation": {},
  "ISSN": [
    "0300-5127",
    "1470-8752"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        2005,
        8,
        1
      ]
    ]
  }
}