DOI: 10.1042/bsr20120042. The endocrine disruptor mono-(2-ethylhexyl)phthalate promotes adipocyte differentiation and induces obesity in mice

The endocrine disruptor mono-(2-ethylhexyl)phthalate promotes adipocyte differentiation and induces obesity in mice

10.1042/bsr20120042

Crossref journal-article

Portland Press Ltd.

Bioscience Reports (288)

Abstract

The environmental obesogen hypothesis proposes that exposure to endocrine disruptors during developmental ‘window’ contributes to adipogenesis and the development of obesity. MEHP [mono-(2-ethylhexyl) phthalate], a metabolite of the widespread plasticizer DEHP [di-(2-ethylhexyl) phthalate], has been found in exposed organisms and identified as a selective PPARγ (peroxisome-proliferator-activated receptor γ) modulator. However, implication of MEHP on adipose tissue development has been poorly investigated. In the present study, we show the dose-dependent effects of MEHP on adipocyte differentiation and GPDH (glycerol-3-phosphate dehydrogenase) activity in the murine 3T3-L1 cell model. MEHP induced the expression of PPARγ as well as its target genes required for adipogenesis in vitro. Moreover, MEHP perturbed key regulators of adipogenesis and lipogenic pathway in vivo. In utero exposure to a low dose of MEHP significantly increased b.w. (body weight) and fat pad weight in male offspring at PND (postnatal day) 60. In addition, serum cholesterol, TAG (triacylglycerol) and glucose levels were also significantly elevated. These results suggest that perinatal exposure to MEHP may be expected to increase the incidence of obesity in a sex-dependent manner and can act as a potential chemical stressor for obesity and obesity-related disorders.

Bibliography

Hao, C., Cheng, X., Xia, H., & Ma, X. (2012). The endocrine disruptor mono-(2-ethylhexyl)phthalate promotes adipocyte differentiation and induces obesity in mice. Bioscience Reports, 32(6), 619â629.

Authors 4

Chanjuan Hao (first)
Xuejia Cheng (additional)
Hongfei Xia (additional)
Xu Ma (additional)

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Dates

Type	When
Created	12 years, 11 months ago (Sept. 7, 2012, 7:49 a.m.)
Deposited	3 years, 9 months ago (Nov. 16, 2021, 1:41 p.m.)
Indexed	2 days, 9 hours ago (Aug. 31, 2025, 7:16 p.m.)
Issued	12 years, 10 months ago (Oct. 19, 2012)
Published	12 years, 10 months ago (Oct. 19, 2012)
Published Online	12 years, 10 months ago (Oct. 19, 2012)
Published Print	12 years, 9 months ago (Dec. 1, 2012)

Funders 0

None

BibTeX

@article{Hao_2012, title={The endocrine disruptor mono-(2-ethylhexyl)phthalate promotes adipocyte differentiation and induces obesity in mice}, volume={32}, ISSN={1573-4935}, url={http://dx.doi.org/10.1042/bsr20120042}, DOI={10.1042/bsr20120042}, number={6}, journal={Bioscience Reports}, publisher={Portland Press Ltd.}, author={Hao, Chanjuan and Cheng, Xuejia and Xia, Hongfei and Ma, Xu}, year={2012}, month=oct, pages={619–629} }

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  "abstract": "<jats:p>The environmental obesogen hypothesis proposes that exposure to endocrine disruptors during developmental \u2018window\u2019 contributes to adipogenesis and the development of obesity. MEHP [mono-(2-ethylhexyl) phthalate], a metabolite of the widespread plasticizer DEHP [di-(2-ethylhexyl) phthalate], has been found in exposed organisms and identified as a selective PPAR\u03b3 (peroxisome-proliferator-activated receptor \u03b3) modulator. However, implication of MEHP on adipose tissue development has been poorly investigated. In the present study, we show the dose-dependent effects of MEHP on adipocyte differentiation and GPDH (glycerol-3-phosphate dehydrogenase) activity in the murine 3T3-L1 cell model. MEHP induced the expression of PPAR\u03b3 as well as its target genes required for adipogenesis in\u00a0vitro. Moreover, MEHP perturbed key regulators of adipogenesis and lipogenic pathway in\u00a0vivo. In utero exposure to a low dose of MEHP significantly increased b.w. (body weight) and fat pad weight in male offspring at PND (postnatal day) 60. In addition, serum cholesterol, TAG (triacylglycerol) and glucose levels were also significantly elevated. These results suggest that perinatal exposure to MEHP may be expected to increase the incidence of obesity in a sex-dependent manner and can act as a potential chemical stressor for obesity and obesity-related disorders.</jats:p>",
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