The Golgi-associated long coiled-coil protein NECC1 participates in the control of the regulated secretory pathway in PC12 cells
10.1042/bj20110554
Crossref journal-article
Portland Press Ltd.
Biochemical Journal (288)
Abstract

Golgi-associated long coiled-coil proteins, often referred to as golgins, are involved in the maintenance of the structural organization of the Golgi apparatus and the regulation of membrane traffic events occurring in this organelle. Little information is available on the contribution of golgins to Golgi function in cells specialized in secretion such as endocrine cells or neurons. In the present study, we characterize the intracellular distribution as well as the biochemical and functional properties of a novel long coiled-coil protein present in neuroendocrine tissues, NECC1 (neuroendocrine long coiled-coil protein 1). The present study shows that NECC1 is a peripheral membrane protein displaying high stability to detergent extraction, which distributes across the Golgi apparatus in neuroendocrine cells. In addition, NECC1 partially localizes to post-Golgi carriers containing secretory cargo in PC12 cells. Overexpression of NECC1 resulted in the formation of juxtanuclear aggregates together with a slight fragmentation of the Golgi and a decrease in K+-stimulated hormone release. In contrast, NECC1 silencing did not alter Golgi architecture, but enhanced K+-stimulated hormone secretion in PC12 cells. In all, the results of the present study identify NECC1 as a novel component of the Golgi matrix and support a role for this protein as a negative modulator of the regulated trafficking of secretory cargo in neuroendocrine cells.

Bibliography

Cruz-García, D., Díaz-Ruiz, A., Rabanal-Ruiz, Y., Peinado, J. R., Gracia-Navarro, F., Castaño, J. P., Montero-Hadjadje, M., Tonon, M.-C., Vaudry, H., Anouar, Y., Vázquez-Martínez, R., & Malagón, M. M. (2012). The Golgi-associated long coiled-coil protein NECC1 participates in the control of the regulated secretory pathway in PC12 cells. Biochemical Journal, 443(2), 387–396.

Authors 12
  1. David Cruz-García (first)
  2. Alberto Díaz-Ruiz (additional)
  3. Yoana Rabanal-Ruiz (additional)
  4. Juan R. Peinado (additional)
  5. Francisco Gracia-Navarro (additional)
  6. Justo P. Castaño (additional)
  7. Maité Montero-Hadjadje (additional)
  8. Marie-Christine Tonon (additional)
  9. Hubert Vaudry (additional)
  10. Youssef Anouar (additional)
  11. Rafael Vázquez-Martínez (additional)
  12. María M. Malagón (additional)
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Dates
Type When
Created 13 years, 7 months ago (Jan. 19, 2012, 5:15 a.m.)
Deposited 3 years, 9 months ago (Nov. 22, 2021, 4:23 p.m.)
Indexed 11 months, 4 weeks ago (Sept. 8, 2024, 11:03 a.m.)
Issued 13 years, 5 months ago (March 27, 2012)
Published 13 years, 5 months ago (March 27, 2012)
Published Online 13 years, 5 months ago (March 27, 2012)
Published Print 13 years, 4 months ago (April 15, 2012)
Funders 0

None

@article{Cruz_Garc_a_2012, title={The Golgi-associated long coiled-coil protein NECC1 participates in the control of the regulated secretory pathway in PC12 cells}, volume={443}, ISSN={1470-8728}, url={http://dx.doi.org/10.1042/bj20110554}, DOI={10.1042/bj20110554}, number={2}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={Cruz-García, David and Díaz-Ruiz, Alberto and Rabanal-Ruiz, Yoana and Peinado, Juan R. and Gracia-Navarro, Francisco and Castaño, Justo P. and Montero-Hadjadje, Maité and Tonon, Marie-Christine and Vaudry, Hubert and Anouar, Youssef and Vázquez-Martínez, Rafael and Malagón, María M.}, year={2012}, month=mar, pages={387–396} }