DOI: 10.1042/bj20071399. Structure of the A20 OTU domain and mechanistic insights into deubiquitination

Structure of the A20 OTU domain and mechanistic insights into deubiquitination

10.1042/bj20071399

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

The NF-κB (nuclear factor κB) regulator A20 antagonises IKK [IκB (inhibitor of κB) kinase] activation by modulating Lys63-linked polyubiquitination of cytokine-receptor-associated factors including TRAF2/6 (tumour-necrosis-factor-receptor-associated factor 2/6) and RIP1 (receptor-interacting protein 1). In the present paper we describe the crystal structure of the N-terminal OTU (ovarian tumour) deubiquitinase domain of A20, which differs from other deubiquitinases but shares the minimal catalytic core with otubain-2. Analysis of conserved surface regions allows prediction of ubiquitin-binding sites for the proximal and distal ubiquitin molecules. Structural and biochemical analysis suggests a novel architecture of the catalytic triad, which might be present in a subset of OTU domains including Cezanne and TRABID (TRAF-binding domain). Biochemical analysis shows a preference of the isolated A20 OTU domain for Lys48-linked tetraubiquitin in vitro suggesting that additional specificity factors might be required for the physiological function of A20 in cells.

Bibliography

Komander, D., & Barford, D. (2007). Structure of the A20 OTU domain and mechanistic insights into deubiquitination. Biochemical Journal, 409(1), 77â85.

Authors 2

David Komander (first)
David Barford (additional)

References 33 Referenced 160

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Dates

Type	When
Created	17 years, 8 months ago (Dec. 13, 2007, 6:17 a.m.)
Deposited	3 years, 9 months ago (Nov. 22, 2021, 10:53 a.m.)
Indexed	4 months, 3 weeks ago (April 11, 2025, 8:26 p.m.)
Issued	17 years, 8 months ago (Dec. 11, 2007)
Published	17 years, 8 months ago (Dec. 11, 2007)
Published Online	17 years, 8 months ago (Dec. 11, 2007)
Published Print	17 years, 8 months ago (Jan. 1, 2008)

Funders 0

None

BibTeX

@article{Komander_2007, title={Structure of the A20 OTU domain and mechanistic insights into deubiquitination}, volume={409}, ISSN={1470-8728}, url={http://dx.doi.org/10.1042/bj20071399}, DOI={10.1042/bj20071399}, number={1}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={Komander, David and Barford, David}, year={2007}, month=dec, pages={77–85} }

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      "URL": "https://portlandpress.com/biochemj/article/409/1/77/43716/Structure-of-the-A20-OTU-domain-and-mechanistic"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2007,
        12,
        11
      ]
    ]
  },
  "references-count": 33,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          2008,
          1,
          1
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1042/BJ20071399",
  "relation": {},
  "ISSN": [
    "0264-6021",
    "1470-8728"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        2007,
        12,
        11
      ]
    ]
  }
}