DOI: 10.1042/bj20060555. The centaurin γ-1 GTPase-like domain functions as an NTPase

The centaurin γ-1 GTPase-like domain functions as an NTPase

10.1042/bj20060555

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

Centaurins are a family of proteins that contain GTPase-activating protein domains, with the γ family members containing in addition a GTPase-like domain. Centaurins reside mainly in the nucleus and are known to activate phosphoinositide 3-kinase, a key regulator of cell proliferation, motility and vesicular trafficking. In the present study, using X-ray structural analysis, enzymatic assays and nucleotide-binding studies, we show that, for CENTG1 (centaurin γ-1) the GTPase-like domain has broader trinucleotide specificity. Alterations within the G4 motif of CENTG1 from the highly conserved NKXD found in typical GTPases to TQDR result in the loss of specificity, a lower affinity for the nucleotides and higher turnover rates. These results indicate that the centaurins could be more accurately classified as NTPases and point to alternative mechanisms of cell signalling control.

Bibliography

Soundararajan, M., Yang, X., Elkins, J. M., Sobott, F., & Doyle, D. A. (2007). The centaurin Î³-1 GTPase-like domain functions as an NTPase. Biochemical Journal, 401(3), 679â688.

Authors 5

Meera Soundararajan (first)
Xiaowen Yang (additional)
Jonathan M. Elkins (additional)
Frank Sobott (additional)
Declan A. Doyle (additional)

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Dates

Type	When
Created	18 years, 5 months ago (March 15, 2007, 5:12 a.m.)
Deposited	3 years, 9 months ago (Nov. 22, 2021, 8:19 a.m.)
Indexed	1 year ago (Aug. 11, 2024, 12:28 p.m.)
Issued	18 years, 7 months ago (Jan. 12, 2007)
Published	18 years, 7 months ago (Jan. 12, 2007)
Published Online	18 years, 7 months ago (Jan. 12, 2007)
Published Print	18 years, 6 months ago (Feb. 1, 2007)

Funders 0

None

BibTeX

@article{Soundararajan_2007, title={The centaurin γ-1 GTPase-like domain functions as an NTPase}, volume={401}, ISSN={1470-8728}, url={http://dx.doi.org/10.1042/bj20060555}, DOI={10.1042/bj20060555}, number={3}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={Soundararajan, Meera and Yang, Xiaowen and Elkins, Jonathan M. and Sobott, Frank and Doyle, Declan A.}, year={2007}, month=jan, pages={679–688} }

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