DOI: 10.1042/bj20040280. Modular organization of a Cdc6-like protein from the crenarchaeon <i>Sulfolobus solfataricus</i>

Modular organization of a Cdc6-like protein from the crenarchaeon Sulfolobus solfataricus

10.1042/bj20040280

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

In the present paper, we report that a Cdc6 (cell-division control)-like factor from the hyperthermophilic crenarchaeon Sulfolobus solfataricus (referred to as SsoCdc6-2) has a modular organization of its biological functions. A reliable model of the SsoCdc6-2 three-dimensional structure was built up, based on the significant sequence identity with the Pyrobaculum aerophylum Cdc6 (PaeCdc6), whose crystallographic structure is known. This allowed us to design two truncated forms of SsoCdc6-2: the ΔC (residues 1–297, molecular mass 35 kDa) and the ΔN (residues 298–400, molecular mass 11 kDa) proteins. The ΔC protein contains the nucleotide-binding Rossmann fold and the Sensor-2 motif (Domains I and II in the PaeCdc6 structure), and retains the ability to bind and hydrolyse ATP. On the other hand, the ΔN protein contains the C-terminal WH (winged helix)-fold (Domain III), and is able to bind DNA molecules and to inhibit the DNA helicase activity of the SsoMCM (mini-chromosome maintenance) complex, although with lesser efficiency with respect to the full-sized SsoCdc6-2. These results provide direct biochemical evidence that the Cdc6 WH-domain is responsible for DNA-binding and inhibition of MCM DNA helicase activity.

Bibliography

DEÂ FELICE, M., ESPOSITO, L., PUCCI, B., DEÂ FALCO, M., MANCO, G., ROSSI, M., & PISANI, F. M. (2004). Modular organization of a Cdc6-like protein from the crenarchaeon Sulfolobus solfataricus. Biochemical Journal, 381(3), 645â653.

Authors 7

Mariarita DE FELICE (first)
Luca ESPOSITO (additional)
Biagio PUCCI (additional)
Mariarosaria DE FALCO (additional)
Giuseppe MANCO (additional)
Mosè ROSSI (additional)
Francesca M. PISANI (additional)

References 29 Referenced 15

{'key': '2021112214575573200_B1', 'first-page': '471', 'article-title': 'Replication mechanisms and operations', 'volume-title': 'DNA replication', 'author': 'Kornberg', 'year': '1992', 'edition': '2nd edn.'} / DNA replication / Replication mechanisms and operations by Kornberg (1992)
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Dates

Type	When
Created	21 years ago (July 27, 2004, 5:59 a.m.)
Deposited	3 years, 8 months ago (Nov. 22, 2021, 2:41 p.m.)
Indexed	1 year, 5 months ago (March 4, 2024, 11:45 a.m.)
Issued	21 years ago (July 27, 2004)
Published	21 years ago (July 27, 2004)
Published Online	21 years ago (July 27, 2004)
Published Print	21 years ago (Aug. 1, 2004)

Funders 0

None

BibTeX

@article{DE_FELICE_2004, title={Modular organization of a Cdc6-like protein from the crenarchaeon Sulfolobus solfataricus}, volume={381}, ISSN={1470-8728}, url={http://dx.doi.org/10.1042/bj20040280}, DOI={10.1042/bj20040280}, number={3}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={DE FELICE, Mariarita and ESPOSITO, Luca and PUCCI, Biagio and DE FALCO, Mariarosaria and MANCO, Giuseppe and ROSSI, Mosè and PISANI, Francesca M.}, year={2004}, month=jul, pages={645–653} }

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