DOI: 10.1042/bc20040508. DSP1, a <i>Drosophila</i> HMG protein, is involved in spatiotemporal expression of the homoeotic gene <i>Sex combs reduced</i>

DSP1, a Drosophila HMG protein, is involved in spatiotemporal expression of the homoeotic gene Sex combs reduced

10.1042/bc20040508

Crossref journal-article

Wiley

Biology of the Cell (311)

Abstract

Background information. The Pc‐G (Polycomb group) and trx‐G (trithorax group) genes play a key role in the regulation of the homoeotic genes. The homoeotic gene Scr (Sex combs reduced) contained in the Antennapedia complex specifies segmental identity of the labial and prothoracic segments in Drosophila. Regulation of Scr requires the action of different enhancer elements spread over several kilobases. We previously identified an HMGB (high mobility group)‐like protein DSP1 (dorsal switch protein 1), which works like a trx‐G protein for the normal Scr expression.Results. In the present study, we attempted to characterize the regulatory sequences involved in the maintenance of the Scr activation by DSP1. We report here, using a transgenic line for the Scr10.0XbaI‐regulatory element, that lack of DSP1 affects the function of a reporter gene in legs' imaginal discs but not in embryos. We show by immunolocalization that DSP1 is recruited on polytene chromosomes to the insertion site of the transgene. Moreover, using chromatin immunoprecipitation experiments, we identify two regions of 1 kb in Scr10.0XbaI as the main DSP1 targets.Conclusion. These results provide strong evidence that the Scr gene expression is influenced by direct interaction between DSP1 and two Scr regulation elements. In addition, our results show that this interaction undergoes dynamic changes during development.

Bibliography

Rappailles, A., Decoville, M., & Locker, D. (2005). DSP1, a Drosophila HMG protein, is involved in spatiotemporal expression of the homoeotic gene Sex combs reduced. Biology of the Cell, 97(10), 779â785. Portico.

Authors 3

Aurélien Rappailles (first)
Martine Decoville (additional)
Daniel Locker (additional)

References 21 Referenced 14

{'key': 'e_1_2_7_2_1', 'first-page': '354', 'volume-title': 'Methods in Cell Biology, vol. 44', 'author': 'Andrew D.J.', 'year': '1994'} / Methods in Cell Biology, vol. 44 by Andrew D.J. (1994)
{'key': 'e_1_2_7_3_1', 'volume-title': 'Drosophila: A Laboratory Manual', 'author': 'Ashburner M.', 'year': '1989'} / Drosophila: A Laboratory Manual by Ashburner M. (1989)
10.1093/genetics/157.1.237 / Genetics / DSP1, an HMG‐like protein, is involved in the regulation of homeotic genes by Decoville M. (2001)
10.1006/dbio.2001.0255
10.1242/dev.126.17.3905
10.1002/gene.10173
10.1093/genetics/139.2.797
10.1093/genetics/139.2.781 / Genetics / Characterization of the cis‐regulatory region of the Drosophila homeotic gene Sex combs reduced by Gindhart J.G. (1995)
10.1242/dev.129.6.1339 / Development / Zeste maintains repression of Ubx transgenes: support for a new model of Polycomb repression by Hur M.W. (2002)
10.1016/S0065-2660(08)60029-2
{'key': 'e_1_2_7_12_1', 'first-page': '640', 'article-title': 'Clusters of master control genes regulate the development of higher organisms', 'volume': '64', 'author': 'Lewis E.B.', 'year': '1993', 'journal-title': 'Curr. Sci.'} / Curr. Sci. / Clusters of master control genes regulate the development of higher organisms by Lewis E.B. (1993)
{'key': 'e_1_2_7_13_1', 'first-page': '403', 'article-title': 'The bithorax complex: the first fifty years', 'volume': '42', 'author': 'Lewis E.B.', 'year': '1998', 'journal-title': 'Int. J. Dev. Biol.'} / Int. J. Dev. Biol. / The bithorax complex: the first fifty years by Lewis E.B. (1998)
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{'key': 'e_1_2_7_18_1', 'first-page': '334', 'volume-title': 'Methods in Cell Biology, vol. 44', 'author': 'Pardue M.L.', 'year': '1994'} / Methods in Cell Biology, vol. 44 by Pardue M.L. (1994)
10.1242/dev.128.1.75 / Development / Recruitment of components of Polycomb group chromatin complexes in Drosophila by Poux S. (2001)
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{'key': 'e_1_2_7_21_1', 'first-page': '455', 'article-title': 'Mapping DNA target sites of chromatin proteins in vivo by formaldehyde crosslinking', 'volume': '119', 'author': 'Strutt H.', 'year': '1999', 'journal-title': 'Methods Mol. Biol.'} / Methods Mol. Biol. / Mapping DNA target sites of chromatin proteins in vivo by formaldehyde crosslinking by Strutt H. (1999)
10.1093/emboj/16.12.3621

Dates

Type	When
Created	19 years, 10 months ago (Sept. 26, 2005, 10:46 a.m.)
Deposited	1 year, 10 months ago (Oct. 11, 2023, 6:18 p.m.)
Indexed	2 months, 4 weeks ago (May 28, 2025, 4:28 a.m.)
Issued	19 years, 10 months ago (Oct. 1, 2005)
Published	19 years, 10 months ago (Oct. 1, 2005)
Published Online	13 years, 7 months ago (Jan. 9, 2012)
Published Print	19 years, 10 months ago (Oct. 1, 2005)

Funders 0

None

BibTeX

@article{Rappailles_2005, title={DSP1, a Drosophila HMG protein, is involved in spatiotemporal expression of the homoeotic gene Sex combs reduced}, volume={97}, ISSN={1768-322X}, url={http://dx.doi.org/10.1042/bc20040508}, DOI={10.1042/bc20040508}, number={10}, journal={Biology of the Cell}, publisher={Wiley}, author={Rappailles, Aurélien and Decoville, Martine and Locker, Daniel}, year={2005}, month=oct, pages={779–785} }

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  "abstract": "<jats:p><jats:italic>Background information</jats:italic>. The <jats:italic>Pc</jats:italic>\u2010G (<jats:italic>Polycomb</jats:italic> group) and <jats:italic>trx</jats:italic>\u2010G (<jats:italic>trithorax</jats:italic> group) genes play a key role in the regulation of the homoeotic genes. The homoeotic gene <jats:italic>Scr</jats:italic> (<jats:italic>Sex combs reduced</jats:italic>) contained in the <jats:italic>Antennapedia complex</jats:italic> specifies segmental identity of the labial and prothoracic segments in <jats:italic>Drosophila</jats:italic>. Regulation of <jats:italic>Scr</jats:italic> requires the action of different enhancer elements spread over several kilobases. We previously identified an HMGB (high mobility group)\u2010like protein DSP1 (dorsal switch protein 1), which works like a trx\u2010G protein for the normal <jats:italic>Scr</jats:italic> expression.</jats:p><jats:p><jats:italic>Results</jats:italic>. In the present study, we attempted to characterize the regulatory sequences involved in the maintenance of the <jats:italic>Scr</jats:italic> activation by DSP1. We report here, using a transgenic line for the <jats:italic>Scr</jats:italic>10.0<jats:italic>Xba</jats:italic>I\u2010regulatory element, that lack of DSP1 affects the function of a reporter gene in legs' imaginal discs but not in embryos. We show by immunolocalization that DSP1 is recruited on polytene chromosomes to the insertion site of the transgene. Moreover, using chromatin immunoprecipitation experiments, we identify two regions of 1 kb in <jats:italic>Scr</jats:italic>10.0<jats:italic>Xba</jats:italic>I as the main DSP1 targets.</jats:p><jats:p><jats:italic>Conclusion</jats:italic>. These results provide strong evidence that the <jats:italic>Scr</jats:italic> gene expression is influenced by direct interaction between DSP1 and two <jats:italic>Scr</jats:italic> regulation elements. In addition, our results show that this interaction undergoes dynamic changes during development.</jats:p>",
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