DOI: 10.1039/c2tx20006f. A comparative study of cellular uptake and cytotoxicity of multi-walled carbon nanotubes, graphene oxide, and nanodiamond

A comparative study of cellular uptake and cytotoxicity of multi-walled carbon nanotubes, graphene oxide, and nanodiamond

10.1039/c2tx20006f

Crossref journal-article

Oxford University Press (OUP)

Toxicology Research (286)

Abstract

AbstractInvestigations of the interactions between carbon nanomaterials (CNMs) and living organisms and their subsequent biological responses are of fundamental significance for toxicity assessment and further biomedical applications. In this work, the cellular uptake and cytotoxicity of multi-walled carbon nanotubes (MWCNTs), graphene oxide (GO) and nanodiamond (ND) were examined and compared. We demonstrated that all of the CNMs were readily internalized by HeLa cells through nonspecific cellular uptake. Their cell uptake ratios showed significant differences in the following order: ND &gt; MWCNTs &gt; GO. A series of biological assays were used to evaluate the cytotoxicity of CNMs. It was found that CNMs showed dose- and time-dependent cytotoxicity to HeLa cells. However, cytotoxicity of CNMs was not associated with their cell uptake ratios. Among them, ND exhibited the highest cell uptake ratio and the least cytotoxicity. To the best of our knowledge, this is the first study which has quantitatively determined and compared the cell uptake ratios and cytotoxicities of MWCNTs, GO and ND. And we expect that these results described here could provide useful information for the development of new strategies to design efficient drug delivery nanocarriers and therapeutic systems as well as deep insights into the mechanism of CNMs' cytotoxicity.

Bibliography

Zhang, X., Hu, W., Li, J., Tao, L., & Wei, Y. (2012). A comparative study of cellular uptake and cytotoxicity of multi-walled carbon nanotubes, graphene oxide, and nanodiamond. Toxicology Research, 1(1), 62â68.

Authors 5

Xiaoyong Zhang (first)
Wenbing Hu (first)
Jing Li (first)
Lei Tao (first)
Yen Wei (first)

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Dates

Type	When
Created	13 years, 4 months ago (April 16, 2012, 6:30 a.m.)
Deposited	2 years, 2 months ago (June 21, 2023, 9:04 p.m.)
Indexed	1 day, 22 hours ago (Aug. 23, 2025, 1:07 a.m.)
Issued	13 years, 3 months ago (May 11, 2012)
Published	13 years, 3 months ago (May 11, 2012)
Published Online	13 years, 3 months ago (May 11, 2012)
Published Print	13 years, 1 month ago (July 1, 2012)

Funders 0

None

BibTeX

@article{Zhang_2012, title={A comparative study of cellular uptake and cytotoxicity of multi-walled carbon nanotubes, graphene oxide, and nanodiamond}, volume={1}, ISSN={2045-4538}, url={http://dx.doi.org/10.1039/c2tx20006f}, DOI={10.1039/c2tx20006f}, number={1}, journal={Toxicology Research}, publisher={Oxford University Press (OUP)}, author={Zhang, Xiaoyong and Hu, Wenbing and Li, Jing and Tao, Lei and Wei, Yen}, year={2012}, month=may, pages={62–68} }

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