Rapid endosomal escape of prickly nanodiamonds: implications for gene delivery
10.1038/srep11661
Crossref journal-article
Springer Science and Business Media LLC
Scientific Reports (297)
Abstract

AbstractThe prickly nanodiamonds easily entered cells via endocytosis followed by unique intracellular translocation characteristics—quick endosomal escape followed by stable residence in cytoplasm. Endosomal membrane rupturing is identified as the major route of nanodiamonds’ escaping the vesicle confinement and to the cytoplasm. Little cytotoxicity is observed to associate with the nanodiamonds’ cytosolic release. Such features enable its application for gene delivery, which requires both effective cellular uptake and cytosolic release of the gene. Taking green fluorescent protein gene as an example, we demonstrate the successful cytosolic delivery and expression of such a gene using the prickly nanodiamonds as carrier.

Bibliography

Chu, Z., Miu, K., Lung, P., Zhang, S., Zhao, S., Chang, H.-C., Lin, G., & Li, Q. (2015). Rapid endosomal escape of prickly nanodiamonds: implications for gene delivery. Scientific Reports, 5(1).

Authors 8
  1. Zhiqin Chu (first)
  2. Kaikei Miu (additional)
  3. Pingsai Lung (additional)
  4. Silu Zhang (additional)
  5. Saisai Zhao (additional)
  6. Huan-Cheng Chang (additional)
  7. Ge Lin (additional)
  8. Quan Li (additional)
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Dates
Type When
Created 10 years, 1 month ago (June 30, 2015, 6:13 a.m.)
Deposited 2 years, 7 months ago (Jan. 5, 2023, 8:59 a.m.)
Indexed 1 month, 1 week ago (July 14, 2025, 11:34 p.m.)
Issued 10 years, 1 month ago (June 30, 2015)
Published 10 years, 1 month ago (June 30, 2015)
Published Online 10 years, 1 month ago (June 30, 2015)
Funders 0

None

@article{Chu_2015, title={Rapid endosomal escape of prickly nanodiamonds: implications for gene delivery}, volume={5}, ISSN={2045-2322}, url={http://dx.doi.org/10.1038/srep11661}, DOI={10.1038/srep11661}, number={1}, journal={Scientific Reports}, publisher={Springer Science and Business Media LLC}, author={Chu, Zhiqin and Miu, Kaikei and Lung, Pingsai and Zhang, Silu and Zhao, Saisai and Chang, Huan-Cheng and Lin, Ge and Li, Quan}, year={2015}, month=jun }