DOI: 10.1038/sj.embor.embor796. Long‐patch DNA repair synthesis during base excision repair in mammalian cells

Long‐patch DNA repair synthesis during base excision repair in mammalian cells

10.1038/sj.embor.embor796

Crossref journal-article

Springer Science and Business Media LLC

EMBO reports (297)

Abstract

The base excision repair (BER) process removes base damage such as oxidation, alkylation or abasic sites. Two BER sub‐pathways have been characterized using in vitro methods, and have been classified according to the length of the repair patch as either ‘short‐patch’ BER (one nucleotide) or ‘long‐patch’ BER (LP‐BER; more than one nucleotide). To investigate the occurrence of LP‐BER in vivo, we developed an assay using a plasmid containing a single modified base in the transcribed strand of the enhanced green fluorescent protein (EGFP) gene and a stop codon, based on a single‐nucleotide mismatch, at varying distances on the 3′ side of the lesion. The reversion of the stop codon occurs after DNA repair synthesis and restores EGFP expression after transfection of mismatch‐repair‐deficient cells. Repair patches longer than one nucleotide were observed for 55–80% or 80–100% of the plasmids with a mean length of 2–6 or 6–12 nucleotides for 8‐oxo‐7,8‐dihydroguanine or a synthetic abasic site, respectively. These data show the existence of LP‐BER in vivo, and emphasize the effect of the type of BER substrate lesion on both the yield and the extent of the LP‐BER sub‐pathway.

Bibliography

Sattler, U., Frit, P., Salles, B., & Calsou, P. (2003). Longâpatch DNA repair synthesis during base excision repair in mammalian cells. EMBO Reports, 4(4), 363â367. Portico.

Authors 4

Ulrike Sattler (first)
Philippe Frit (additional)
Bernard Salles (additional)
Patrick Calsou (additional)

References 25 Referenced 61

Dates

Type	When
Created	22 years, 4 months ago (April 9, 2003, 1:10 p.m.)
Deposited	1 year, 8 months ago (Dec. 18, 2023, 3:57 p.m.)
Indexed	1 month, 1 week ago (July 23, 2025, 8:35 a.m.)
Issued	22 years, 5 months ago (March 7, 2003)
Published	22 years, 5 months ago (March 7, 2003)
Published Online	22 years, 5 months ago (March 7, 2003)
Published Print	22 years, 5 months ago (April 1, 2003)

Funders 0

None

BibTeX

@article{Sattler_2003, title={Long‐patch DNA repair synthesis during base excision repair in mammalian cells}, volume={4}, ISSN={1469-3178}, url={http://dx.doi.org/10.1038/sj.embor.embor796}, DOI={10.1038/sj.embor.embor796}, number={4}, journal={EMBO reports}, publisher={Springer Science and Business Media LLC}, author={Sattler, Ulrike and Frit, Philippe and Salles, Bernard and Calsou, Patrick}, year={2003}, month=mar, pages={363–367} }

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